Geiser Dawn L, Mayo Jonathan J, Winzerling Joy J
Department of Nutritional Science, College of Agriculture and Life Sciences and Center for Insect Science, University of Arizona, 1177 East 4th Street, Shantz Building, Room 405, Tucson, AZ 85721-0038, USA.
Insect Biochem Mol Biol. 2007 May;37(5):418-29. doi: 10.1016/j.ibmb.2007.01.003. Epub 2007 Jan 25.
Mosquitoes must blood feed in order to complete their life cycle. The blood meal provides a high level of iron that is required for egg development. We are interested in developing control strategies that interfere with this process. We report the temporal effects of iron exposure on iron metabolism of Aedes aegypti larval cells. These cells take up iron in linear relationship to exposure time and distribute the iron primarily to the membranes. Iron uptake increases cytoplasmic, membrane and secreted ferritin. Membrane ferritin is abundant in cells treated with iron, increases in cells in the absence of iron exposure and is associated with the secretory pathway. Our data suggest that in contrast to mammals, mosquitoes control intracellular iron levels by producing membrane ferritin in anticipation of an iron load such as that provided by a blood meal and support the hypothesis that secreted ferritin is a primary iron storage protein for these animals.
蚊子必须吸食血液才能完成其生命周期。血餐提供了卵子发育所需的高水平铁。我们有兴趣开发干扰这一过程的控制策略。我们报告了铁暴露对埃及伊蚊幼虫细胞铁代谢的时间效应。这些细胞摄取铁的量与暴露时间呈线性关系，并将铁主要分布到细胞膜。铁摄取增加了细胞质、细胞膜和分泌型铁蛋白。膜铁蛋白在用铁处理的细胞中含量丰富，在未暴露于铁的细胞中也会增加，并且与分泌途径相关。我们的数据表明，与哺乳动物不同，蚊子通过在预期有血餐提供的铁负荷时产生膜铁蛋白来控制细胞内铁水平，并支持分泌型铁蛋白是这些动物主要铁储存蛋白的假说。
