Department of Pathogenic Biology, Jining Medical College, Jining, Shandong Province 272067, People's Republic of China.
Parasitol Res. 2012 Feb;110(2):939-59. doi: 10.1007/s00436-011-2580-4. Epub 2011 Aug 14.
The complete sequence of transferrin has been cloned from cypermethrin-resistant strain of Culex pipiens pallens(Cr-C strain). Quantitative reverse transcription PCR analysis indicated that the transferrin transcription level was 12.6 times higher in Cr-C strain than in susceptible strain at fourth instar larvae. The transferrin expression was also found to be consistently higher throughout the life cycle of Cr-C strain. A protein of predicted size 90.8 kDa has been detected by Western blotting in transferrin-transfected mosquito C6/36 cells. These transferrin-transfected cells also showed enhanced cypermethrin resistance compared to null-transfected or plasmid vector-transfected cells as determined by methyl tritiated thymidine((3)H-TdR) incorporation. These results indicate that transferrin is expressed at higher levels in Cr-C strain and may confer some insecticide resistance in C. pipiens pallens.
已从拟除虫菊酯抗性白纹伊蚊（Cr-C 株）中克隆出转铁蛋白的完整序列。定量反转录 PCR 分析表明，在第四龄幼虫期，Cr-C 株的转铁蛋白转录水平比敏感株高 12.6 倍。在 Cr-C 株的整个生命周期中，转铁蛋白的表达也一直较高。通过 Western blot 在转铁蛋白转染的蚊子 C6/36 细胞中检测到预测大小为 90.8 kDa 的蛋白质。与空转染或质粒载体转染的细胞相比，这些转铁蛋白转染的细胞在甲硫氨酸氚化胸腺嘧啶核苷（(3)H-TdR）掺入测定中也显示出增强的拟除虫菊酯抗性。这些结果表明，Cr-C 株中转铁蛋白的表达水平较高，可能赋予白纹伊蚊一定的杀虫剂抗性。
