Qiao Li, Zhou Yu-Zhi, Qi Xiu-Lan, Lin Li-Hong, Chen Huan, Pang Li-Yan, Pei Yue-Hu
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University.
J Antibiot (Tokyo). 2007 Apr;60(4):261-4. doi: 10.1038/ja.2007.32.
Cunninghamella elegans has been employed for the biotransformation of cinobufagin to afford 5 metabolites. The structures of the transformation products have been characterized as 12alpha-hydroxybufagin, 11alpha-hydroxybufagin, 12beta-hydroxy-desacetylcinobufagin, 3-oxo-12alpha-hydroxybufagin and 12beta-hydroxybufagin. Products 12alpha-hydroxybufagin and 11alpha-hydroxybufagin are new compounds. In vitro both the biotransformation products and cinobufagin all showed cytotoxic activities against HeLa cells.
