Lebedev V P, Bilichenko S V, Ordyan N E, Pivina S G, Nechiporenko S P, Puzyrev A A, Mikheeva E A, Kubacheva K K
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, 6 Makarov Bank, 199034 St. Petersburg, Russia.
Neurosci Behav Physiol. 2007 May;37(4):341-7. doi: 10.1007/s11055-007-0019-2.
Studies on rats with experimental diabetes induced by administration of alloxan showed that transcranial electrostimulation of endorphinergic brain structures stimulates the regeneration of damaged beta-cells in pancreatic islets of Langerhans. This was identified on pancreatic sections stained with hematoxylin and eosin. De novo formation of small islets was noted, as evidenced by their regeneration from progenitor cells. After transcranial electrostimulation, islet beta-cells stained by the Gomori method showed recovery of granularity - a sign of insulin production. Application of an immunoenzyme method demonstrated recovery of blood insulin levels, the dynamics of increases in which showed a highly significant negative correlation with a decrease in blood glucose. These data led to the conclusion that the antihyperglycemic effect of transcranial electrostimulation in experimental alloxan diabetes results from reparative regeneration of beta-cells in islets of Langerhans with recovery of their insulin-producing function.
对通过注射四氧嘧啶诱导实验性糖尿病的大鼠进行的研究表明，经颅电刺激内啡肽能脑结构可刺激受损的胰岛β细胞在胰岛中再生。这在苏木精和伊红染色的胰腺切片上得到了证实。观察到有新形成的小胰岛，这由祖细胞再生得到证明。经颅电刺激后，用Gomori方法染色的胰岛β细胞显示颗粒度恢复——这是胰岛素产生的标志。免疫酶法的应用证明了血液胰岛素水平的恢复，其升高动态与血糖降低呈高度显著的负相关。这些数据得出结论，经颅电刺激在实验性四氧嘧啶糖尿病中的降血糖作用是由于胰岛β细胞的修复性再生及其胰岛素分泌功能的恢复。
