Cho Eun Jae, Hwang Hye Jin, Kim Sang Woo, Oh Jung Young, Baek Yu Mi, Choi Jang Won, Bae Song Hwan, Yun Jong Won
Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Republic of Korea.
Appl Microbiol Biotechnol. 2007 Jul;75(6):1257-65. doi: 10.1007/s00253-007-0972-2. Epub 2007 Apr 25.
The anti-diabetic activities of the exopolysaccharides (EPS) produced by submerged mycelial culture of two different mushrooms, Tremella fuciformis and Phellinus baumii, in ob/ob mice were investigated. All the animals were randomly divided into three groups with seven animals in each group: The control group received 0.9% NaCl solution; the diabetic groups were treated with EPS from T. fuciformis (Tf EPS) and P. baumii (Pb EPS) at the level of 200 mg/kg body weight using an oral zoned daily for 52 days. The plasma glucose levels in the EPS-fed mice were substantially reduced by about 52% (Tf EPS) and 32% (Pb EPS), respectively, as compared to control mice. The results of oral glucose tolerance test (OGTT) revealed that both EPS-fed groups significantly increased the glucose disposal after 52 days of EPS treatments. Furthermore, higher food efficiency ratios and reduced blood triglyceride levels were observed in the EPS-treated groups. Because peroxisome proliferator-activated receptor gamma (PPAR-gamma) is indeed a key regulator of insulin action, we investigated the expression pattern of adipose tissue PPAR-gamma messenger RNA (mRNA) and plasma levels of PPAR-gamma. It was revealed that PPAR-gamma was significantly activated in response to EPS treatments. The results suggested that both EPS exhibited considerable hypoglycemic effect and improved insulin sensitivity possibly through regulating PPAR-gamma-mediated lipid metabolism. Our results indicated that two mushroom-derived EPS might be developed as potential oral hypoglycemic agents or functional foods for the management of non-insulin-dependent diabetes mellitus.
研究了两种不同蘑菇——银耳和鲍氏层孔菌——深层菌丝体培养产生的胞外多糖（EPS）对ob/ob小鼠的抗糖尿病活性。所有动物随机分为三组，每组七只：对照组接受0.9%氯化钠溶液；糖尿病组分别用200mg/kg体重的银耳EPS（Tf EPS）和鲍氏层孔菌EPS（Pb EPS）进行口服给药，每天一次，持续52天。与对照小鼠相比，喂食EPS的小鼠血浆葡萄糖水平分别显著降低了约52%（Tf EPS）和32%（Pb EPS）。口服葡萄糖耐量试验（OGTT）结果显示，在进行52天的EPS治疗后，两个喂食EPS的组的葡萄糖处置能力均显著提高。此外，在EPS治疗组中观察到更高的食物效率比和降低的血液甘油三酯水平。由于过氧化物酶体增殖物激活受体γ（PPAR-γ）确实是胰岛素作用的关键调节因子，我们研究了脂肪组织PPAR-γ信使核糖核酸（mRNA）的表达模式和PPAR-γ的血浆水平。结果显示，PPAR-γ在EPS治疗后被显著激活。结果表明，两种EPS均表现出相当大的降血糖作用，并可能通过调节PPAR-γ介导的脂质代谢改善胰岛素敏感性。我们的结果表明，两种蘑菇来源的EPS可能被开发为潜在的口服降糖药物或功能性食品，用于管理非胰岛素依赖型糖尿病。
