Barrios-Llerena Martin E, Burja Adam M, Wright Phillip C
Biological and Environmental Systems Group, Department of Chemical and Process Engineering, University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK.
J Ind Microbiol Biotechnol. 2007 Jun;34(6):443-56. doi: 10.1007/s10295-007-0216-6. Epub 2007 Apr 25.
Molecular screening using degenerate PCR to determine the presence of secondary metabolite genes in cyanobacteria was performed. This revealed 18 NRPS and 19 PKS genes in the 21 new cyanobacterial strains examined, representing three families of cyanobacteria (Nostocales, Chroococales and Oscillatoriales). A BLAST analysis shows that these genes have similarities to known cyanobacterial natural products. Analysis of the NRPS adenylation domain indicates the presence of novel features previously ascribed to both proteobacteria and cyanobacteria. Furthermore, binding-pocket predictions reveal diversity in the amino acids used during the biosynthesis of compounds. A similar analysis of the PKS ketosynthase domain shows significant structural diversity and their presence in both mixed modules with NRPS domains and individually as part of a PKS module. We have been able to classify the NRPS genes on the basis of their binding-pockets. Further, we show how this data can be used to begin to link structure to function by an analysis of the compounds Scyptolin A and Hofmannolin from Scytonema sp. PCC 7110.
利用简并PCR进行分子筛选，以确定蓝藻中次生代谢物基因的存在。在所检测的21株新蓝藻菌株中发现了18个非核糖体肽合成酶（NRPS）基因和19个聚酮合酶（PKS）基因，这些菌株代表了三个蓝藻目（念珠藻目、色球藻目和颤藻目）。BLAST分析表明，这些基因与已知的蓝藻天然产物具有相似性。对NRPS腺苷化结构域的分析表明，存在先前归因于变形菌和蓝藻的新特征。此外，结合口袋预测揭示了化合物生物合成过程中使用的氨基酸的多样性。对PKS酮合成酶结构域的类似分析显示出显著的结构多样性，并且它们存在于与NRPS结构域的混合模块中，以及单独作为PKS模块的一部分。我们已经能够根据其结合口袋对NRPS基因进行分类。此外，我们通过分析来自席藻属PCC 7110的化合物Scyptolin A和Hofmannolin，展示了这些数据如何用于开始将结构与功能联系起来。
