Pietruska M, Zak J, Pietruski J, Wysocka J
Department of Periodontal and Oral Mucosa Diseases, Medical University of Białystok, Poland.
Adv Med Sci. 2006;51 Suppl 1:166-9.
Lipopolysaccharides (LPS), a major component of the cell membrane of gram-negative bacteria, are the main stimulants of the host immune response, initiating inflammatory changes and responsible for periodontal tissue destruction. The mCD14, which is found primarily on monocytes and macrophages, is the key membranous receptor involved in LPS binding. CD14 is also present in the serum as a soluble form (sCD14) released due to shedding from monocytes. The aim of the study was to assess CD14 expression on peripheral blood monocytes in patients with generalized aggressive periodontitis (GAP). The level of sCD14 was also determined in the serum of GAP patients.
The study group consisted of 16 patients with generalized aggressive periodontitis, the control group had 13 systemically and periodontally healthy subjects. The expression of mCD14 was determined by flow cytometry and expressed as mean intensity of fluorescence (MIF). Serum sCD14 level was examined with ELISA method.
The expressions of mCD14 on monocytes in GAP patients and control subjects were comparable. No statistically significant differences were noted in the mean serum sCD14 level between GAP and control subjects.
As periodontitis is a local disorder affecting a small fragment of the oral cavity it seems likely that chronic bacterial infection existing there is not reflected in the peripheral parameters.
脂多糖（LPS）是革兰氏阴性菌细胞膜的主要成分，是宿主免疫反应的主要刺激物，引发炎症变化并导致牙周组织破坏。主要存在于单核细胞和巨噬细胞上的mCD14是参与LPS结合的关键膜受体。CD14也以可溶性形式（sCD14）存在于血清中，它是由于单核细胞脱落而释放的。本研究的目的是评估广泛侵袭性牙周炎（GAP）患者外周血单核细胞上CD14的表达情况。同时也测定了GAP患者血清中sCD14的水平。
研究组由16例广泛侵袭性牙周炎患者组成，对照组有13例全身和牙周健康的受试者。通过流式细胞术测定mCD14的表达，并以平均荧光强度（MIF）表示。采用ELISA法检测血清sCD14水平。
GAP患者和对照组单核细胞上mCD14的表达相当。GAP患者和对照组之间的血清sCD14平均水平无统计学显著差异。
由于牙周炎是一种影响口腔小部分区域的局部疾病，口腔中存在的慢性细菌感染似乎未在外周参数中体现出来。
