Rodriguez Virginia, Bartolomé Borja, Armisén Margarita, Vidal Carmen
Department of Allergy, Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain.
Ann Allergy Asthma Immunol. 2007 Apr;98(4):393-6. doi: 10.1016/S1081-1206(10)60888-5.
Sea urchins are increasingly used as culinary products in Japanese and Korean cuisine throughout the world.
To investigate a possible IgE-mediated allergy in a patient who experienced an anaphylactic reaction after eating sea urchin roe and mussels.
A 40-year-old man experienced generalized pruritus and urticaria, shortness of breath, and wheezing 10 minutes after eating steamed mussels and boiled sea urchin roe. Investigations included skin prick tests, serum specific IgE determinations, oral challenge tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting.
Results of skin prick tests with common aeroallergens were negative. Positive prick test responses were obtained with boiled and raw sea urchin extracts, boiled and raw sea urchins themselves, and boiled sea urchin water. Results of an oral challenge test with steamed mussels were negative. Five minutes after the oral mucosa contacted the boiled sea urchin extract, lip pruritus was present. Specific IgE concentrations to boiled sea urchin water and boiled and raw sea urchins surpassed the criterion for a positive result (1.1, 0.6, and 0.4 kU/L, respectively). In boiled sea urchin extract, a 118-kDa band appeared as the unique relevant IgE-binding antigen.
In this patient with IgE-mediated food allergy to sea urchin roe, a 118-kDa protein seemed to be the antigen responsible for the reaction.
海胆在全球范围内越来越多地被用作日本和韩国料理的烹饪食材。
调查一名在食用海胆籽和贻贝后发生过敏反应的患者是否存在可能由IgE介导的过敏。
一名40岁男性在食用清蒸贻贝和水煮海胆籽10分钟后出现全身瘙痒、荨麻疹、呼吸急促和喘息。检查包括皮肤点刺试验、血清特异性IgE测定、口服激发试验、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹。
常见气传变应原的皮肤点刺试验结果为阴性。水煮和生海胆提取物、水煮和生海胆本身以及水煮海胆水的点刺试验结果为阳性。清蒸贻贝的口服激发试验结果为阴性。口腔黏膜接触水煮海胆提取物5分钟后出现唇部瘙痒。水煮海胆水、水煮和生海胆的特异性IgE浓度超过阳性结果标准（分别为1.1、0.6和0.4 kU/L）。在水煮海胆提取物中，一条118 kDa的条带作为唯一相关的IgE结合抗原出现。
在这名对海胆籽有IgE介导的食物过敏的患者中，一种118 kDa的蛋白质似乎是导致该反应的抗原。
