Tanaka Kenichi, Kondo Yasuto, Inuo Chisato, Nakajima Yoichi, Tsuge Ikuya, Doi Satoru, Yanagihara Shigeto, Yoshikawa Tetsushi, Urisu Atsuo
Department of Pediatrics, The Second Teaching Hospital, Fujita Health University, Nagoya, Japan.
Int Arch Allergy Immunol. 2014;164(3):222-7. doi: 10.1159/000365728. Epub 2014 Aug 16.
Sea urchin roe can cause anaphylactic reactions the first time they are consumed; therefore, careful clinical attention should be paid to their effects. However, no previous study has examined the allergens in sea urchin roe using sera from more than one patient. We attempted to identify sea urchin allergens using sera from 5 patients with sea urchin allergies.
We enrolled 5 patients with relevant medical histories, positive results on a skin prick test and/or a food challenge test, and high levels of sea urchin-specific IgE in an enzyme-linked immunosorbent assay. We performed SDS-PAGE, immunoblotting, immunoblot inhibition, and N-terminal amino acid sequence detection.
Ten protein bands ranging from 18 to 170 kDa were detected in more than 2 patients' sera. In immunoblotting, the protein band for the 170-kDa major yolk protein was recognized by 4 of the 5 sera. Furthermore, the reaction between IgE and the protein band for egg cortical vesicle protein (18 kDa) was inhibited by the addition of salmon roe extract.
Major yolk protein was confirmed to be one of the main allergens in sea urchin roe. In addition, egg cortical vesicle protein (18 kDa) was demonstrated to be an important protein for cross-reactivity with salmon roe.
海胆籽初次食用时可引起过敏反应；因此，应密切关注其临床影响。然而，此前尚无研究使用多名患者的血清检测海胆籽中的过敏原。我们试图用5名对海胆过敏患者的血清来鉴定海胆过敏原。
我们招募了5名有相关病史、皮肤点刺试验和/或食物激发试验结果呈阳性、且在酶联免疫吸附测定中血清海胆特异性IgE水平较高的患者。我们进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹、免疫印迹抑制和N端氨基酸序列检测。
在2名以上患者的血清中检测到10条分子量在18至170 kDa之间的蛋白条带。在免疫印迹中，5份血清中有4份识别出170 kDa主要卵黄蛋白的蛋白条带。此外，添加鲑鱼籽提取物可抑制IgE与卵皮质颗粒蛋白（18 kDa）蛋白条带之间的反应。
主要卵黄蛋白被确认为海胆籽中的主要过敏原之一。此外，卵皮质颗粒蛋白（18 kDa）被证明是与鲑鱼籽发生交叉反应的一种重要蛋白。
