Poirier Laurence, Amiard Jean-Claude, Mondeguer Florence, Quiniou Françoise, Ruiz Nicolas, Pouchus Yves François, Montagu Monique
Université de Nantes, Nantes Atlantique Universités, SMAB, EA2160, Faculté de pharmacie, 1 rue G. Veil-BP 53508, Nantes F-44000, France.
J Chromatogr A. 2007 Aug 10;1160(1-2):106-13. doi: 10.1016/j.chroma.2007.04.006. Epub 2007 Apr 12.
Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.
