Tuméo E, Gbaguidi-Haore H, Patry I, Bertrand X, Thouverez M, Talon D
Service d'Hygiène Hospitalière, CHU Jean Minjoz, 25030 Besançon Cedex, France.
Int J Hyg Environ Health. 2008 Mar;211(1-2):200-4. doi: 10.1016/j.ijheh.2007.02.010. Epub 2007 Apr 24.
Previous reports have studied the presence of antibiotic-resistant Pseudomonas aeruginosa strains in hospital wastewater without determination of their clonal relationship with the clinical strains of this species. The objectives of this study were to quantify the presence of P. aeruginosa in wastewater of our hospital, to determine their antibiotic-resistance profile and to potentially trace clinical antibiotic-resistant strains from patients to wastewater. Specimens were taken at the end of the wastewater network of our hospital just before the reject in the collective network of the town. Two specimens were taken each Monday during 12 weeks. All P. aeruginosa isolates recovered from hospitalised patients during the study period were collected. Genotyping of both clinical and wastewater isolates was determined by using pulsed-field gel electrophoresis (PFGE). The antibiotic-resistance profile of wastewater isolates was different from that of clinical isolates. The mechanisms involved in antibiotic resistance were different according to the origin of the isolates (wastewater versus human isolates). There was no common PFGE pattern in antibiotic-resistant P. aeruginosa from humans and wastewater. This study suggests that the risk of spread of antibiotic resistance in hospital wastewater is limited.
以往的报告研究了医院废水中耐抗生素铜绿假单胞菌菌株的存在情况，但未确定其与该菌种临床菌株的克隆关系。本研究的目的是量化我院废水中铜绿假单胞菌的存在情况，确定其抗生素耐药谱，并有可能追踪从患者到废水的临床耐抗生素菌株。样本在我院废水网络末端、即将排入城镇公共网络之前采集。在12周内，每周一采集两份样本。收集了研究期间从住院患者中分离出的所有铜绿假单胞菌。临床和废水分离株的基因分型通过脉冲场凝胶电泳（PFGE）确定。废水分离株的抗生素耐药谱与临床分离株不同。根据分离株的来源（废水与人类分离株），抗生素耐药的机制也不同。来自人类和废水的耐抗生素铜绿假单胞菌没有共同的PFGE模式。本研究表明，医院废水中抗生素耐药性传播的风险是有限的。
