Herrmann M E, Talpos G B, Mohamed A N, Saxe A, Ratner S, Lalley P A, Wolman S R
Michigan Cancer Foundation, Detroit.
Surgery. 1991 Dec;110(6):941-7; discussion 947-8.
Tissue from nine patients with malignant tumors and two with benign tumors was cultured briefly before cytogenetic analysis. The tumors included one goiter and one Hürthle cell adenoma, one lymphoma, one medullary carcinoma, two Hürthle cell cancers, and five papillary cancers, varying widely in clinical staging and histologic differentiation. When assessed, DNA content was aneuploid in two of six malignant tumors. Various culture conditions (oxygen levels, dissociation methods, and media) were evaluated; the end points were growth, cell differentiation, and time to first harvest. Clonal aberrations were detected in one of four successfully harvested papillary cancers: they consisted of trisomy 7 and a rearrangement of chromosome 10. The rea (10) seen in 22 of 27 cells involved bands q11-21. Two other papillary tumors and a medullary cancer (a family member with multiple endocrine neoplasia type IIA) showed tetraploidy and nonclonal numerically aberrant cells. A lymphoma and two benign lesions showed no cytogenetic abnormality. The tumor with rea (10) is of special interest because abnormalities of 10q have been reported repeatedly in thyroid tumors, including two cases of papillary thyroid tumors with a structural aberration similar to that of the presented case. This rearrangement could affect the ret-proto-oncogene, localized to 10q11.2 which is activated in some papillary thyroid carcinomas.
在进行细胞遗传学分析之前，对9例恶性肿瘤患者和2例良性肿瘤患者的组织进行了短期培养。这些肿瘤包括1例甲状腺肿、1例许特莱细胞腺瘤、1例淋巴瘤、1例髓样癌、2例许特莱细胞癌和5例乳头状癌，临床分期和组织学分化差异很大。评估时，6例恶性肿瘤中有2例的DNA含量为非整倍体。对各种培养条件（氧气水平、解离方法和培养基）进行了评估；终点指标为生长、细胞分化和首次收获时间。在4例成功收获的乳头状癌中有1例检测到克隆性畸变：包括7号染色体三体和10号染色体重排。在27个细胞中的22个细胞中观察到的重排（10）涉及q11-21带。另外2例乳头状肿瘤和1例髓样癌（1例患有IIA型多发性内分泌腺瘤的家庭成员）显示四倍体和非克隆性数量异常细胞。1例淋巴瘤和2例良性病变未显示细胞遗传学异常。具有重排（10）的肿瘤特别令人关注，因为10q异常在甲状腺肿瘤中已被反复报道，包括2例乳头状甲状腺肿瘤，其结构畸变与本病例相似。这种重排可能影响定位于10q11.2的原癌基因ret，该基因在一些乳头状甲状腺癌中被激活。
