Yildirim Zuhal, Uçgun N Irem, Kiliç Nedret, Gürsel E, Sepici-Dinçel Aylin
Gazi University, Faculty of Medicine, Department of Medical Biochemistry, 06510 Beşevler, Ankara, Turkey.
Ann N Y Acad Sci. 2007 Apr;1100:207-12. doi: 10.1196/annals.1395.020.
To investigate the role of zinc and copper in the development of pseudoexfoliation (PSX) syndrome, 34 cataract patients with PSX syndrome and 27 cataract patients without PSX syndrome were included in the study and groups were matched for age and gender. During the cataract surgery, lenses were obtained intraoperatively, frozen under liquid nitrogen, and kept at -70 degrees C until processing. Zinc and copper concentrations were measured by atomic absorption spectrophotometric method after the homogenization (acid hydrolysis) of dried lenses. The mean concentration of zinc in the lens from patients with PSX (20.33 +/- 8.76 microg/g tissue; range 11.04-42.94 microg/g tissue) was significantly lower than that measured in the lens of patients without PSX (28.88 +/- 15.32 microg/g tissue; range 12.02-64.32 microg/g tissue) (P < 0.05). The mean concentration of copper in the lens from patients with PSX (29.51 +/- 10.05 microg/g tissue; range 12.69-59.71 microg/g tissue) and in the lens of patients without PSX (39.72 +/- 25.64 microg/g tissue; range 12.38-92.14 microg/g tissue) was not statistically different. The decreased content of zinc could increase oxidative stress. The results support the role of oxidative stress in the development of PSX in cataract patients.
为研究锌和铜在假性剥脱（PSX）综合征发病中的作用，本研究纳入了34例患有PSX综合征的白内障患者和27例无PSX综合征的白内障患者，并根据年龄和性别对两组进行了匹配。在白内障手术过程中，术中获取晶状体，置于液氮中冷冻，并保存在-70℃直至处理。干燥晶状体经匀浆（酸水解）后，采用原子吸收分光光度法测定锌和铜的浓度。PSX患者晶状体中锌的平均浓度（20.33±8.76μg/g组织；范围11.04 - 42.94μg/g组织）显著低于无PSX患者晶状体中测得的浓度（28.88±15.32μg/g组织；范围12.02 - 64.32μg/g组织）（P<0.05）。PSX患者晶状体中铜的平均浓度（29.51±10.05μg/g组织；范围12.69 - 59.71μg/g组织）与无PSX患者晶状体中铜的平均浓度（39.72±25.64μg/g组织；范围12.38 - 92.14μg/g组织）无统计学差异。锌含量的降低可能会增加氧化应激。这些结果支持氧化应激在白内障患者PSX发病中的作用。
