Savage S M, Donaldson L A, Cherian S, Chilukuri R, White V A, Sopori M L
Immunotoxicology Section, Lovelace Medical Foundation, Albuquerque, New Mexico 87108.
Toxicol Appl Pharmacol. 1991 Dec;111(3):523-9. doi: 10.1016/0041-008x(91)90256-e.
We have previously reported that chronic exposure of rats to cigarette smoke inhibits the antibody-forming cell (AFC) response to both T-dependent and T-independent antigens and may reflect B cell dysfunction. In this communication we extend these studies to show that T cell functions are normal in chronically smoke-exposed rats (SM) as judged by their responses to mitogens and "nominal" or alloantigens. While B cells from SM respond significantly to the B cell mitogen lipopolysaccharide (LPS), they fail to proliferate in response to anti-IgM (anti-mu) or to produce significant AFC response to sheep red blood cells. On the basis of the number of rosettes formed with trinitrophenylated (TNP) horse red blood cells; the frequency of TNP-binding cells (TNP-ABC) in the spleens of SM is comparable to sham control rats. However, the proliferation of TNP-ABC to TNP-Brucella abortus is significantly decreased in SM. These differences in SM B cell responses, i.e., between LPS and anti-mu/antigen, may to be related to the ability of LPS to bypass a portion of the membrane signal transduction cascade. These results suggest that cigarette smoke affects an early step(s) in the antigen-dependent B cell signal transduction pathway.
我们之前报道过，长期让大鼠接触香烟烟雾会抑制其对T细胞依赖性和T细胞非依赖性抗原的抗体形成细胞（AFC）反应，这可能反映了B细胞功能障碍。在本交流中，我们扩展了这些研究，以表明通过慢性烟雾暴露大鼠（SM）对丝裂原以及“名义”或同种异体抗原的反应判断，其T细胞功能正常。虽然来自SM的B细胞对B细胞丝裂原脂多糖（LPS）有明显反应，但它们对抗IgM（抗μ）无增殖反应，对绵羊红细胞也无显著的AFC反应。根据与三硝基苯基化（TNP）马红细胞形成的玫瑰花结数量判断，SM脾脏中TNP结合细胞（TNP-ABC）的频率与假手术对照大鼠相当。然而，SM中TNP-ABC对TNP-流产布鲁氏菌的增殖明显降低。SM的B细胞反应在LPS和抗μ/抗原之间的这些差异，可能与LPS绕过部分膜信号转导级联反应的能力有关。这些结果表明，香烟烟雾影响抗原依赖性B细胞信号转导途径中的早期步骤。
