Yang Hong-Yi, Qian Shi-Jun, Li Gao-Wo
China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):323-6.
With PVA as the carrier, the frozen beer yeast cells were immobilized for production of CTP from CMP. we explored the optimal condition of the immobilization from the aspects of the type, concentration of the PVA, and the immobilizing methods of cells In all 8 continuous batch of fermentation under the reactional condition of the immobilized cells, the conversion rate of CTP were maintained about 85% - 95%. Moreever, the storage stability of immobilized cells were investigated, and the products was also isolated and identifided by HPLC.
以聚乙烯醇（PVA）为载体，对冷冻啤酒酵母细胞进行固定化，用于从胞苷一磷酸（CMP）生产胞苷三磷酸（CTP）。我们从PVA的类型、浓度以及细胞固定化方法等方面探索了最佳固定化条件。在固定化细胞的反应条件下进行了总共8批次的连续发酵，CTP的转化率保持在约85% - 95%。此外，研究了固定化细胞的储存稳定性，并通过高效液相色谱法（HPLC）对产物进行了分离和鉴定。
