Wang Wen-Guo, Zhao Xiao-Guang, Wang Sheng-Hua, Chen Fang
Department of Key Laboratory of Bio-resources and Eco-environment , Ministry of Education , College of Life Sciences , Sichuan University , Chengdu 610064, China.
Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):337-42.
This paper studied the effect of polyethylene glycol (PEG) on regeneration and free proline accumulation of callus of Pogonatherum paniceum (Lam.) Hack. under motionless liquid culture condition and shake liquid culture condition. Callus of P. paniceum had the ability to resist the stress of PEG. The effects of PEG stress and culture conditions on the callus of P. paniceum appeared mainly in two aspects, delaying regeneration time and debasing regeneration rates. The shake liquid culture mainly delayed the regeneration time and PEG stress mainly debased the regeneration rates. Free proline accumulated in the two culture conditions, and the contents of proline were positively correlated with PEG concentrations and culture time. After stress removal, most of the callus could recover the ability of regeneration, and the free proline might pay an important part in the inhibition and recovery. So it must be chosen a more than 300 g x L(-1) PEG concentration and long than 3 weeks culture time in the selection of drought-resistant mutants of P. paniceum. The motionless liquid culture was more suitable for selection of drought-resistant mutants.
本文研究了聚乙二醇（PEG）在静置液体培养条件和振荡液体培养条件下对金发草愈伤组织再生及游离脯氨酸积累的影响。金发草愈伤组织具有抵抗PEG胁迫的能力。PEG胁迫和培养条件对金发草愈伤组织的影响主要体现在两个方面，即延迟再生时间和降低再生率。振荡液体培养主要延迟再生时间，而PEG胁迫主要降低再生率。在两种培养条件下均有游离脯氨酸积累，脯氨酸含量与PEG浓度和培养时间呈正相关。胁迫解除后，大部分愈伤组织能够恢复再生能力，游离脯氨酸可能在抑制和恢复过程中起重要作用。因此，在金发草抗旱突变体筛选中，必须选择PEG浓度大于300 g·L⁻¹且培养时间超过3周的条件。静置液体培养更适合抗旱突变体的筛选。
