Department of Biology, University of Oulu, PO Box 3000, FIN-90014 Oulu, Finland.
Environ Microbiol Rep. 2010 Aug;2(4):619-24. doi: 10.1111/j.1758-2229.2010.00197.x.
A mycobacterium was isolated from micropropagated Pogonatherum paniceum and identified as a close relative of Mycobacterium cookii. The endophyte diversity in the shoots of potted and micropropagated P. paniceum plants was studied by culture-independent techniques. Group- and strain-specific PCR demonstrated that the P. paniceum plants harboured the isolated Mycobacterium strain as a minority. Altogether 101 clones of the PCR products were sequenced. The shoots of potted P. paniceum plants harboured unculturable endophytes in the families Phyllobacteriaceae, Hyphomicrobiaceae, Sphingobacteriaceae, Enterobacteriaceae, Alcaligenaceae and Mycobacteriaceae. Among the unculturable Mycobacteriaceae strains related to Mycobacterium chubuense, M. poriferae, M. obuense, M. fortuitum, M. neoaurum, M. diernhoferi, M. intracellulare and M. cookii were identified. Three unique sequences that clustered with M. llatzarense and M. mucogenicum were identified in micropropagated plants. According to the results, the shoots and micropropagated tissues of rock plant are inhabited by mycobacteria, which should stimulate further studies on the diversity of unculturable mycobacteria in edible crop plants.
从微繁殖的狼尾草中分离出一支分枝杆菌，并将其鉴定为分枝杆菌属 cookii 的近亲。采用非培养技术研究了盆栽和微繁殖狼尾草植株芽中的内生菌多样性。群组和菌株特异性 PCR 表明，微繁殖的 P. paniceum 植物中仅少数含有分离出的分枝杆菌菌株。总共对 101 个 PCR 产物克隆进行了测序。盆栽 P. paniceum 植物的芽中含有未培养的内生菌，属于 Phyllobacteriaceae、Hyphomicrobiaceae、Sphingobacteriaceae、Enterobacteriaceae、Alcaligenaceae 和分枝杆菌科。在不可培养的分枝杆菌科菌株中，与分枝杆菌属的 M. chubuense、M. poriferae、M. obuense、M. fortuitum、M. neoaurum、M. diernhoferi、M. intracellulare 和 M. cookii 有关的菌株被鉴定出来。在微繁殖植物中鉴定出了三个与 M. llatzarense 和 M. mucogenicum 聚类的独特序列。根据这些结果，石生植物的芽和微繁殖组织中存在分枝杆菌，这应该会激发对可食用作物植物中不可培养分枝杆菌多样性的进一步研究。
