Odamaki T, Xiao J Z, Iwabuchi N, Sakamoto M, Takahashi N, Kondo S, Iwatsuki K, Kokubo S, Togashi H, Enomoto T, Benno Y
Food Research and Development Laboratory Morinaga Milk Industry Co, Ltd, Zama, Kanagawa, Japan.
J Investig Allergol Clin Immunol. 2007;17(2):92-100.
We have previously reported the results of a randomized, double-blind, placebo-controlled trial that found the intake of yogurt supplemented with a probiotic strain, Bifidobacterium longum BB536, alleviates symptoms and affects blood parameters in individuals with Japanese cedar pollinosis (JCPsis) during the pollen season.
In the present study, fecal microbiota were investigated to examine whether any changes occur during the pollen season and whether any influence is exerted by probiotic intake.
Yogurt either with BB536 (BB536 yogurt) or without BB536 (placebo yogurt) was administered for 14 weeks at 2 x 100 g per day to 40 subjects (17 men, 23 women) with a clinical history of JCPsis. Fecal samples were obtained from 23 subjects (placebo group, n=13; BB536 group, n=10) before and during the intervention (weeks 4, 9 and 13) and fecal microbiota were analyzed using terminal-restriction fragment length polymorphism and real-time polymerase chain reaction (PCR) methods.
From the fluctuation patterns of terminal-restriction fragments, the Bacteroides fragilis group and bifidobacteria were among the species that changed most with pollen dispersion. Real-time PCR analyses indicated that the cell numbers of the B fragilis group increased significantly along with pollen dispersion in both BB536 and placebo groups. Cell numbers of bifidobacteria were significantly higher in the BB536 group compared with the placebo group (P < .05 at weeks 4 and 9). The ratio of cell numbers of the B fragilis group to bifidobacteria increased significantly during the pollen season in the placebo group (P < .01 at weeks 9 and 14), but not in the BB536 group. An in vitro study using peripheral blood mononuclear cells from JCPsis subjects indicated that strains of the B fragilis group induced significantly more helper T cell (T(H)) type2 cytokines (interleukin [IL]-6) but fewer T(H)1 cytokines (IL-12 and interferon) compared with those of bifidobacteria.
These results suggest a relationship between fluctuation in intestinal microbiota and pollinosis allergy. Furthermore, intake of BB536 yogurt appears to exert positive ihfluences on the formation of anti-allergic microbiota.
我们之前报道了一项随机、双盲、安慰剂对照试验的结果，该试验发现摄入添加了益生菌长双歧杆菌BB536的酸奶可缓解日本雪松花粉症（JCPsis）患者在花粉季节的症状并影响血液参数。
在本研究中，对粪便微生物群进行调查，以检查在花粉季节是否发生任何变化以及益生菌摄入是否产生任何影响。
将含有BB536的酸奶（BB536酸奶）或不含BB536的酸奶（安慰剂酸奶）以每天2×100 g的剂量给予40名有JCPsis临床病史的受试者（17名男性，23名女性），持续14周。在干预前及干预期间（第4、9和13周）从23名受试者（安慰剂组，n = 13；BB536组，n = 10）获取粪便样本，并使用末端限制性片段长度多态性和实时聚合酶链反应（PCR）方法分析粪便微生物群。
从末端限制性片段的波动模式来看，脆弱拟杆菌群和双歧杆菌是随花粉传播变化最大的物种。实时PCR分析表明，在BB536组和安慰剂组中，脆弱拟杆菌群的细胞数量均随花粉传播显著增加。与安慰剂组相比，BB536组双歧杆菌的细胞数量显著更高（第4周和第9周时P < .05）。在安慰剂组中，花粉季节期间脆弱拟杆菌群与双歧杆菌的细胞数量之比显著增加（第9周和第14周时P < .01），但在BB536组中未增加。一项使用JCPsis受试者外周血单个核细胞的体外研究表明，与双歧杆菌相比，脆弱拟杆菌群的菌株诱导产生的辅助性T细胞（TH）2型细胞因子（白细胞介素[IL]-6）显著更多，但TH1细胞因子（IL-12和干扰素）更少。
这些结果表明肠道微生物群的波动与花粉症过敏之间存在关联。此外，摄入BB536酸奶似乎对抗过敏微生物群的形成产生积极影响。
