Mackey Abigail L, Kjaer Michael, Dandanell Sune, Mikkelsen Kristian H, Holm Lars, Døssing Simon, Kadi Fawzi, Koskinen Satu O, Jensen Charlotte H, Schrøder Henrik D, Langberg Henning
Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Bldg. 8, Bispebjerg Bakke 23, 2400 Copenhagen NV, Denmark.
J Appl Physiol (1985). 2007 Aug;103(2):425-31. doi: 10.1152/japplphysiol.00157.2007. Epub 2007 Apr 26.
The consumption of nonsteroidal anti-inflammatory drugs (NSAIDs) is widespread among athletes when faced with muscle soreness or injury, but the effects of NSAIDs on satellite cell activity in humans are unknown. To investigate this, 14 healthy male endurance athletes (mean peak oxygen consumption 62 ml x kg(-1) x min(-1)) volunteered for the study, which involved running 36 km. They were divided into two groups and received either 100 mg indomethacin per day or placebo. Muscle biopsies collected before the run and on days 1, 3, and 8 afterward were analyzed for satellite cells by immunohistochemistry with the aid of neural cell adhesion molecule (NCAM) and fetal antigen-1 (FA1) antibodies. Muscle biopsies were also collected from untrained individuals for comparison. Compared with preexercise levels, a 27% increase in the number of NCAM+ cells was observed on day 8 postexercise in the placebo group (P < 0.05), while levels remained similar at all time points in the NSAID group. No change was seen in the proportion of FA1+ cells, although lower levels were found in the muscle of endurance-trained athletes compared with untrained individuals (P < 0.05). These results suggest that ingestion of anti-inflammatory drugs attenuates the exercise-induced increase in satellite cell number, supporting the role of the cyclooxygenase pathway in satellite cell activity.
非甾体抗炎药（NSAIDs）在运动员中广泛使用，用于缓解肌肉酸痛或损伤，但NSAIDs对人体卫星细胞活性的影响尚不清楚。为了研究这一问题，14名健康的男性耐力运动员（平均峰值耗氧量62 ml·kg⁻¹·min⁻¹）自愿参与了这项涉及36公里跑步的研究。他们被分为两组，分别每天服用100毫克吲哚美辛或安慰剂。在跑步前以及跑步后第1天、第3天和第8天采集肌肉活检样本，借助神经细胞黏附分子（NCAM）和胎儿抗原-1（FA1）抗体，通过免疫组织化学分析卫星细胞。还从未受过训练的个体中采集肌肉活检样本进行比较。与运动前水平相比，安慰剂组运动后第8天NCAM⁺细胞数量增加了27%（P < 0.05），而NSAIDs组在所有时间点的水平保持相似。FA1⁺细胞的比例没有变化，尽管与未受过训练的个体相比，耐力训练运动员肌肉中的FA1⁺细胞水平较低（P < 0.05）。这些结果表明，摄入抗炎药物会减弱运动诱导的卫星细胞数量增加，支持环氧化酶途径在卫星细胞活性中的作用。
