Roberts Brandon M, Geddis Alyssa V, Sczuroski Cara E, Reynoso Marinaliz, Hughes Julie M, Gwin Jess A, Staab Jeffery S
Military Performance Division, US Army Research Institute of Environmental Medicine, 10 General Greene Ave., Building 42, Natick, MA, 01760, USA.
Military Nutrition Division, US Army Research Institute of Environmental Medicine, 10 General Greene Ave., Building 42, Natick, MA, 01760, USA.
Eur J Appl Physiol. 2024 Dec;124(12):3607-3617. doi: 10.1007/s00421-024-05565-5. Epub 2024 Jul 24.
Non-steroidal anti-inflammatory drugs (NSAIDs) possess analgesic and anti-inflammatory properties by inhibiting cyclooxygenase (COX) enzymes. Conflicting evidence exists on whether NSAIDs influence signaling related to muscle adaptations and exercise with some research finding a reduction in muscle protein synthesis signaling via the AKT-mTOR pathway, changes in satellite cell signaling, reductions in muscle protein degradation, and reductions in cell proliferation. In this study, we determined if a single maximal dose of flurbiprofen (FLU), celecoxib (CEL), ibuprofen (IBU), or a placebo (PLA) affects the short-term muscle signaling responses to plyometric exercise.
This was a block randomized, double-masked, crossover design, where 12 participants performed four plyometric exercise bouts consisting of 10 sets of 10 plyometric jumps at 40% 1RM. Two hours before exercise, participants consumed a single dose of celecoxib (CEL 200 mg), IBU (800 mg), FLU (100 mg) or PLA with food. Muscle biopsy samples were collected before and 3-h after exercise from the vastus lateralis. Data were analyzed using a repeated measures (RM) ANOVA, ANOVA, or a Friedman test. Significance was considered at p < 0.05.
We found no treatment effects on the mRNA expression of PTSG1, PTSG2, MYC, TBP, RPLOP, MYOD1, Pax7, MYOG, Atrogin-1, or MURF1 (all, p > 0.05). We also found no treatment effects on AKT-mTOR signaling or MAPK signaling measured through the phosphorylation status of mTORS2441, mTORS2448, RPS6 235/236, RPS 240/244, 4EBP1, ERK1/2, p38 T180/182 normalized to their respective total abundance (all, p > 0.05). However, we did find a significant difference between MNK1 T197/202 in PLA compared to FLU (p < .05).
A single, maximal dose of IBU, CEL, or FLU taken prior to exercise did not affect the signaling of muscle protein synthesis, protein degradation, or ribosome biogenesis three hours after a plyometric training bout.
非甾体抗炎药（NSAIDs）通过抑制环氧化酶（COX）发挥止痛和抗炎特性。关于NSAIDs是否影响与肌肉适应和运动相关的信号传导，存在相互矛盾的证据。一些研究发现，NSAIDs会通过AKT-mTOR途径减少肌肉蛋白合成信号传导、改变卫星细胞信号传导、减少肌肉蛋白降解以及减少细胞增殖。在本研究中，我们确定单次最大剂量的氟比洛芬（FLU）、塞来昔布（CEL）、布洛芬（IBU）或安慰剂（PLA）是否会影响对增强式运动的短期肌肉信号反应。
这是一项区组随机、双盲、交叉设计，12名参与者进行了4组增强式运动，每组包括10组以40% 1RM进行的10次增强式跳跃。在运动前两小时，参与者随食物服用单次剂量的塞来昔布（CEL 200毫克）、布洛芬（IBU 800毫克）、氟比洛芬（FLU 100毫克）或安慰剂。在运动前和运动后3小时从股外侧肌采集肌肉活检样本。使用重复测量（RM）方差分析、方差分析或Friedman检验对数据进行分析。p < 0.05被认为具有统计学意义。
我们发现，治疗对PTSG1、PTSG2、MYC、TBP、RPLOP、MYOD1、Pax7、MYOG、Atrogin-1或MURF1的mRNA表达没有影响（所有p > 0.05）。我们还发现，通过mTORS2441、mTORS2448、RPS6 235/236、RPS 240/244、4EBP1、ERK1/2、p38 T180/182相对于各自总丰度的磷酸化状态测量的AKT-mTOR信号传导或MAPK信号传导，治疗也没有影响（所有p > 0.05）。然而，我们确实发现，与氟比洛芬相比，安慰剂组中MNK1 T197/202存在显著差异（p < 0.05）。
运动前服用单次最大剂量的布洛芬、塞来昔布或氟比洛芬，在增强式训练后三小时，对肌肉蛋白合成、蛋白降解或核糖体生物发生的信号传导没有影响。
