Gentry T, Foster S, Winstead L, Deibert E, Fiordalisi M, Balber A
Aldagen Inc., Durham, North Carolina 27713, USA.
Cytotherapy. 2007;9(3):259-74. doi: 10.1080/14653240701218516.
ALDH(br) cells express high aldehyde dehydrogenase (ALDH) activity and have progenitor cell activity in several contexts. We characterized human BM ALDH(br) cells to determine whether cell sorting based on ALDH activity isolates potentially useful populations for cell therapy.
We measured the expression of ALDH and cell-surface Ag by flow cytometry and compared the ability of sorted ALDH(br), and BM populations remaining after ALDH(br) cells were removed (ALDH(dim) populations), to develop into several cell lineages in culture.
The ALDH(br) population comprised 1.2+/-0.8% (mean+/-SD, n=30) nucleated cells and was enriched in cells expressing CD34, CD117, CD105, CD127, CD133 and CD166, and in primitive CD34(+) CD38(-) and CD34(+) CD133(+) progenitors. Most of the CD34(+) and CD133(+) cells were ALDH(dim). ALDH(br) populations had 144-fold more hematopoietic colony-forming activity than ALDH(dim) cells and included all megakaryocyte progenitors. ALDH(br) populations readily established endothelial cell monolayers in cultures. Cells generating endothelial colonies in 7 days were 435-fold more frequent in ALDH(br) than ALDH(dim) populations. CFU-F were 9.5-fold more frequent in ALDH(br) than ALDH(dim) cells, and ALDH(br) cells gave rise to multipotential mesenchymal cell cultures that could be driven to develop into adipocytes, osteoblasts and chondrocytes.
Hematopoietic, endothelial and mesenchymal progenitor cells can be isolated simultaneously from human BM by cell sorting based on ALDH activity. BM ALDH(br) populations may be useful in several cell therapy applications.
ALDH(br)细胞在多种情况下表达高醛脱氢酶（ALDH）活性并具有祖细胞活性。我们对人骨髓ALDH(br)细胞进行了表征，以确定基于ALDH活性的细胞分选是否能分离出对细胞治疗可能有用的细胞群体。
我们通过流式细胞术测量了ALDH和细胞表面抗原的表达，并比较了分选的ALDH(br)细胞以及去除ALDH(br)细胞后剩余的骨髓群体（ALDH(dim)群体）在培养中分化为几种细胞谱系的能力。
ALDH(br)群体占核细胞的1.2±0.8%（平均值±标准差，n = 30），富含表达CD34、CD117、CD105、CD127、CD133和CD166的细胞，以及原始的CD34(+)CD38(-)和CD34(+)CD133(+)祖细胞。大多数CD34(+)和CD133(+)细胞是ALDH(dim)。ALDH(br)群体的造血集落形成活性比ALDH(dim)细胞高144倍，并且包括所有巨核细胞祖细胞。ALDH(br)群体在培养中很容易形成内皮细胞单层。在7天内产生内皮集落的细胞在ALDH(br)群体中比在ALDH(dim)群体中频繁435倍。CFU-F在ALDH(br)群体中比在ALDH(dim)细胞中频繁9.5倍，并且ALDH(br)细胞产生了多能间充质细胞培养物，这些培养物可以被诱导分化为脂肪细胞、成骨细胞和软骨细胞。
通过基于ALDH活性的细胞分选可以从人骨髓中同时分离造血、内皮和间充质祖细胞。骨髓ALDH(br)群体可能在多种细胞治疗应用中有用。
