胰腺癌中ADAM 9和ADAM 15信使核糖核酸表达增加。

Increased expression of ADAM 9 and ADAM 15 mRNA in pancreatic cancer.

作者信息

Yamada Daisuke, Ohuchida Kenoki, Mizumoto Kazuhiro, Ohhashi Seiji, Yu Jun, Egami Takuya, Fujita Hayato, Nagai Eishi, Tanaka Masao

机构信息

Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

出版信息

Anticancer Res. 2007 Mar-Apr;27(2):793-9.

PMID:17465204

Abstract

BACKGROUND

A disintegrin and metalloproteases (ADAMs) comprise a multifunctional family of membrane-anchored proteins. ADAM 9 and ADAM 15 are involved in cell migration and invasion. Expression of ADAM 9 and ADAM 15 was reported to be altered in several types of cancer.

MATERIALS AND METHODS

Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure the expression of ADAM 9 mRNA in bulk pancreatic tissues. Results showed no significant difference in the expression of ADAM 9 mRNA between pancreatic cancer and non-neoplastic pancreas. Primary cultured pancreatic fibroblasts also expressed ADAM 9 mRNA. Therefore, a laser microdissection and pressure catapulting technique was employed to isolate cancer cells from tumor tissues. The expression of ADAM 9 and ADAM 15 mRNA was measured in microdissected samples (cancer cells, n = 11; normal epithelial cells, n = 13 for ADAM 9; cancer cells, n = 9; normal epithelial cells, n = 9 for ADAM 15).

RESULTS

Pancreatic cancer cells expressed significantly higher levels of ADAM 9 and ADAM 15 mRNA than did normal pancreatic epithelial cells (p = 0.016 for ADAM 9; p = 0.004 for ADAM 15).

CONCLUSION

ADAM 9 and ADAM 15 are involved in pancreatic cancer. Microdissection-based analysis appears to be indispensable for the accurate analysis of the expression of certain ADAM family members in pancreatic cancer.

摘要

背景

解整合素金属蛋白酶（ADAMs）是一类多功能的膜锚定蛋白家族。ADAM 9和ADAM 15参与细胞迁移和侵袭。据报道，ADAM 9和ADAM 15在几种类型的癌症中表达发生改变。

材料与方法

采用定量实时逆转录-聚合酶链反应检测大块胰腺组织中ADAM 9 mRNA的表达。结果显示，胰腺癌组织与非肿瘤性胰腺组织中ADAM 9 mRNA的表达无显著差异。原代培养的胰腺成纤维细胞也表达ADAM 9 mRNA。因此，采用激光显微切割和压力弹射技术从肿瘤组织中分离癌细胞。检测显微切割样本（ADAM 9：癌细胞，n = 11；正常上皮细胞，n = 13；ADAM 15：癌细胞，n = 9；正常上皮细胞，n = 9）中ADAM 9和ADAM 15 mRNA的表达。