Tucker O N, Dannenberg A J, Yang E K, Zhang F, Teng L, Daly J M, Soslow R A, Masferrer J L, Woerner B M, Koki A T, Fahey T J
Department of Surgery, New York Presbyterian Hospital and Weill Medical College of Cornell University, New York, New York 10021, USA.
Cancer Res. 1999 Mar 1;59(5):987-90.
A large body of evidence suggests that cyclooxygenase-2 (COX-2) is important in gastrointestinal cancer. The purpose of this study was to determine whether COX-2 was expressed in adenocarcinoma of the human pancreas. Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in pancreatic tissue. Levels of COX-2 mRNA were increased by >60-fold in pancreatic cancer compared to adjacent nontumorous tissue. COX-2 protein was present in 9 of 10 cases of adenocarcinoma of the pancreas but was undetectable in nontumorous pancreatic tissue. Immunohistochemical analysis showed that COX-2 was expressed in malignant epithelial cells. In cultured human pancreatic cancer cells, levels of COX-2 mRNA and protein were induced by treatment with tumor-promoting phorbol esters. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of pancreatic cancer.
大量证据表明，环氧化酶-2（COX-2）在胃肠道癌中起重要作用。本研究的目的是确定COX-2是否在人胰腺癌中表达。采用定量逆转录-PCR、免疫印迹和免疫组织化学方法评估胰腺组织中COX-2的表达。与相邻的非肿瘤组织相比，胰腺癌中COX-2 mRNA水平增加了60倍以上。10例胰腺腺癌中有9例存在COX-2蛋白，但在非肿瘤胰腺组织中未检测到。免疫组织化学分析显示COX-2在恶性上皮细胞中表达。在培养的人胰腺癌细胞中，用促肿瘤佛波酯处理可诱导COX-2 mRNA和蛋白水平升高。综上所述，这些结果表明COX-2可能是预防或治疗胰腺癌的靶点。
