Tani Nobuyuki, Ichikawa Daisuke, Ikoma Daito, Tomita Haruhisa, Sai Soujin, Ikoma Hisashi, Okamoto Kazuma, Ochiai Toshiya, Ueda Yuji, Otsuji Eigo, Yamagishi Hisakazu, Miura Norimasa, Shiota Goshi
Department of Surgery, Division of Digestive Surgery, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Anticancer Res. 2007 Mar-Apr;27(2):1207-12.
The diagnostic value of circulating mRNA for the early detection of primary and recurrent gastric cancer was evaluated.
Circulating hTERT and MUC1 mRNA were amplified in the plasma from 52 gastric cancer patients (40 preoperative and 12 postoperative patients) and 20 healthy controls. The results were compared with those of a circulating cancer cell assay and methylation-specific polymerase chain reaction assay.
Cell-free mRNA of the analyzed genes was detected in 6 (15%) preoperative gastric cancer patients (hTERT: 3 and MUC1: 4 patients) and 2 follow-up patients. These mRNAs were not detected in the plasma from healthy volunteers. There was no correlation between the results of the cell-free mRNA and the other assays.
Detection of circulating cell-free mRNA might serve as a new complementary tumor marker for gastric cancer.
评估循环mRNA对原发性和复发性胃癌早期检测的诊断价值。
对52例胃癌患者（40例术前患者和12例术后患者）及20例健康对照者的血浆中循环hTERT和MUC1 mRNA进行扩增。将结果与循环癌细胞检测和甲基化特异性聚合酶链反应检测结果进行比较。
在6例（15%）术前胃癌患者（hTERT：3例，MUC1：4例）和2例随访患者中检测到分析基因的无细胞mRNA。在健康志愿者的血浆中未检测到这些mRNA。无细胞mRNA结果与其他检测结果之间无相关性。
循环无细胞mRNA的检测可能成为胃癌新的补充肿瘤标志物。
