Jung Ronald E, Hämmerle Christoph H, Kokovic Vladimir, Weber Franz E
Department of Fixed and Removable Prosthodontics and Dental Material Science, University of Zurich, Switzerland.
Int J Oral Maxillofac Implants. 2007 Mar-Apr;22(2):258-66.
The aim of the present study was to test whether a newly developed synthetic matrix made of polyethylene-glycol (PEG) containing a covalently bound peptide of the parathyroid hormone (PTH1-34) enhances bone regeneration compared to grafting procedures and to spontaneous healing.
In each of 16 rabbits used, 4 titanium cylinders were screwed into perforated slits made in the cortical bone of the calvaria. The cylinders were either left empty (control) or filled with 1 of the following: (1) PEG matrix and hydroxyapatite/tricalcium phosphate (HA/TCP) granules, (2) PEG matrix containing 100microg/mL of PTH and HA/TCP granules, or (3) PEG matrix containing 20microg/mL of PTH1-34 and HA/TCP granules. After 8 weeks, the animals were sacrificed, and ground sections were obtained for histology.
Quantitative histomorphometry demonstrated a significantly increased amount of newly formed bone for PTH1-34 compared to sites treated with PEG and HA/TCP and to empty control sites (P < .01; analysis of variance and subsequent pairwise Student t test). The mean percentages of mineralized bone were 19.6%+/-6.0% for 100 microg/mL PTH, 18.0% +/- 6.2% for 20microg/mL PTH, 12.0% +/-6.5% for PEG and HA/TCP without PTH, and 10.5% +/-3.7% for the empty control. The mean areas of bone regenerated within the cylinders were 53.5% +/-22.7% for 100 microg/mL PTH, 51.1% +/-22.6% for 20 microg/mL PTH, 34.3% +/- 22.5% for PEG and HA/TCP without PTH, and 23.2% +/-10.1% for the empty control.
Human and animal trials have demonstrated that daily systemic injection of PTH increases bone mineral density. The present study showed that local administration of PTH was also effective in stimulating bone formation.
It is concluded that this synthetic PEG hydrogel containing a covalently bound peptide of the PTH combined with HA/TCP granules significantly stimulated in situ bone augmentation in rabbits.
本研究旨在测试一种新开发的由聚乙二醇（PEG）制成的合成基质，其含有共价结合的甲状旁腺激素（PTH1-34）肽，与移植手术和自发愈合相比，是否能增强骨再生。
在16只实验用兔中，每只兔在颅骨皮质骨上的穿孔狭缝中拧入4个钛圆柱体。圆柱体要么保持空的（对照），要么填充以下物质之一：（1）PEG基质和羟基磷灰石/磷酸三钙（HA/TCP）颗粒；（2）含有100μg/mL PTH的PEG基质和HA/TCP颗粒；（3）含有20μg/mL PTH1-34的PEG基质和HA/TCP颗粒。8周后，处死动物，获取磨片进行组织学检查。
定量组织形态计量学显示，与用PEG和HA/TCP处理的部位以及空对照部位相比，PTH1-34处理部位新形成的骨量显著增加（P <.01；方差分析及随后的成对学生t检验）。100μg/mL PTH的矿化骨平均百分比为19.6%±6.0%，20μg/mL PTH为18.0%±6.2%，不含PTH的PEG和HA/TCP为12.0%±6.5%，空对照为10.5%±3.7%。圆柱体内再生骨的平均面积，100μg/mL PTH为53.5%±22.7%，20μg/mL PTH为51.1%±22.6%，不含PTH的PEG和HA/TCP为34.3%±22.5%，空对照为23.2%±10.1%。
人体和动物试验表明，每日全身注射PTH可增加骨矿物质密度。本研究表明，局部应用PTH在刺激骨形成方面也有效。
得出结论，这种含有共价结合的PTH肽与HA/TCP颗粒的合成PEG水凝胶能显著刺激兔原位骨增大。
