糖尿病大鼠与正常大鼠视网膜蛋白表达的差异。

Differences in expression of retinal proteins between diabetic and normal rats.

作者信息

Liu Shang-Qing, Kang Jian, Li Cheng-Jun, Tang En-Jie, Wen Bin, Cai Rong, Yang Hui-Jun

机构信息

Department of Anatomy, School of Preclinical and Forensic Medicine, Sichuan University, 17 Renming Nan Road, Chengdu 610041, Sichuan Province, China.

出版信息

World J Gastroenterol. 2007 Apr 14;13(14):2118-24. doi: 10.3748/wjg.v13.i14.2118.

DOI:10.3748/wjg.v13.i14.2118

PMID:17465459

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4319136/

Abstract

AIM

To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes.

METHODS

Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (P<0.05) were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics.

RESULTS

2-DE showed that the expression was up-regulated in 5 retinal proteins, down-regulated in 23 retinal proteins, and disappeared in 8 retinal proteins. Eight spots were identified from the 36 spots by tandem mass spectrometry (MS/MS) and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells. Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far.

CONCLUSION

There are differences in expression of retinal proteins between diabetic and normal rats. These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes.

摘要

目的

比较并鉴定正常大鼠与糖尿病大鼠视网膜蛋白表达的差异，分析糖尿病所致视网膜疾病的分子发病机制。

方法

采用二维聚丙烯酰胺凝胶电泳（2-DE）观察糖尿病大鼠和正常大鼠视网膜组织蛋白表达的变化。随机选取一些表现出统计学显著差异（P<0.05）的蛋白点，通过串联质谱进行鉴定，并进行生物信息学分析。

结果

2-DE显示，5种视网膜蛋白表达上调，23种视网膜蛋白表达下调，8种视网膜蛋白表达消失。通过串联质谱（MS/MS）从36个蛋白点中鉴定出8个，并进行生物信息学分析。鸟苷酸激酶1、磷酸丙糖异构酶1、ATP合酶亚基d、白蛋白和二甲基精氨酸二甲胺水解酶2在信号转导中起重要作用。磷酸丙糖异构酶1、αB晶状体蛋白、ATP合酶亚基d和过氧化物酶体增殖物激活受体6参与视网膜组织的能量代谢。鸟苷酸激酶1在视网膜视杆光感受器细胞的光激发中起重要作用。迄今为止，βA1晶状体蛋白在糖尿病视网膜中是否起作用尚不清楚。