Turek Z, Černý V, Pařízková R
University Hospital Hradec Králové, Department of Anesthesiology and Intensive Care Medicine, Hradec Králové, Czech Republic.
Physiol Res. 2008;57(3):365-371. doi: 10.33549/physiolres.931137. Epub 2007 Apr 25.
The pathophysiology of microcirculation is intensively investigated to understand disease development at the microscopic level. Orthogonal polarization spectral (OPS) imaging and its successor sidestream dark-field (SDF) imaging are relatively new noninvasive optical techniques allowing direct visualization of microcirculation in both clinical and experimental studies. The goal of this experimental study was to describe basic microcirculatory parameters of skeletal muscle and ileal serous surface microcirculation in the rat using SDF imaging and to standardize the technical aspects of the protocol. Interindividual variability in functional capillary density (FCD) and small vessels (<25 microm in diameter) proportion was determined in anesthetized rats on the surface of quadriceps femoris (m. rectus femoris and m. vastus medialis) and serous surface of ileum. Special custom made flexible arm was used to fix the SDF probe minimizing the pressure movement artifacts. Clear high contrast images were analyzed off-line. The mean FCD obtained from the surface of skeletal muscle and ileal serous surface was 219 (213-225 cm/cm(2)) and 290 (282-298 cm/cm(2)) respectively. There was no statistically significant difference between rats in mean values of FCD obtained from the muscle (P = 0.273) in contrast to ileal serous surface, where such difference was statistically significant (P = 0.036). No statistically significant differences in small vessels percentage was detected on either the muscle surface (P = 0.739) or on ileal serous surface (P = 0.659). Our study has shown that interindividual variability of basic microcirculatory parameters in rat skeletal muscle and ileum is acceptable when using SDF imaging technique according to a highly standardized protocol and with appropriate fixation device. SDF imaging represents promising technology for experimental and clinical studies.
为了在微观层面理解疾病发展，人们对微循环的病理生理学进行了深入研究。正交偏振光谱（OPS）成像及其后续的侧流暗视野（SDF）成像，是相对较新的非侵入性光学技术，可在临床和实验研究中直接观察微循环。本实验研究的目的是使用SDF成像描述大鼠骨骼肌和回肠浆膜表面微循环的基本参数，并规范该方案的技术细节。在麻醉大鼠的股四头肌（股直肌和股内侧肌）表面和回肠浆膜表面，测定功能毛细血管密度（FCD）和小血管（直径<25微米）比例的个体间变异性。使用特制的柔性臂固定SDF探头，以尽量减少压力移动伪影。离线分析清晰的高对比度图像。从骨骼肌表面和回肠浆膜表面获得的平均FCD分别为219（213 - 225 cm/cm²）和290（282 - 298 cm/cm²）。与回肠浆膜表面相比，从肌肉获得的FCD平均值在大鼠之间无统计学显著差异（P = 0.273），而回肠浆膜表面的这种差异具有统计学显著性（P = 0.036）。在肌肉表面（P = 0.739）或回肠浆膜表面（P = 0.659），小血管百分比均未检测到统计学显著差异。我们的研究表明，根据高度标准化的方案并使用适当的固定装置，采用SDF成像技术时，大鼠骨骼肌和回肠基本微循环参数的个体间变异性是可以接受的。SDF成像代表了一种用于实验和临床研究的有前景的技术。
