Department of Gerontology and Metabolism, Charles University in Prague, Czech Republic.
Physiol Res. 2011;60(1):75-81. doi: 10.33549/physiolres.931937. Epub 2010 Oct 15.
Assessment of the cerebral microcirculation by on-line visualization has been impossible for a long time. Sidestream dark-field (SDF) imaging is a relatively new method allowing direct visualization of cerebral surface layer microcirculation using hand-held probe for direct contact with target tissue. The aim of this study was to elucidate the feasibility of studying the cerebral microcirculation in situ by SDF imaging and to assess the basic cerebral microcirculatory parameters in mechanically ventilated rabbits. Images were obtained using SDF imaging from the surface of the brain via craniotomy. Clear high contrast SDF images were successfully obtained. Total small-vessel density was 14.6+/-1.8 mm/mm(2), total all-vessel density was 17.9+/-1.7 mm/mm(2), DeBacker score was 12.0+/-1.6 mm(-1) and microvascular flow index was 3.0+/-0.0. This method seems to be applicable in animal studies with possibility to use SDF imaging also intraoperatively, providing unique opportunity to study cerebral microcirculation during various experimental and clinical settings.
很长一段时间以来，通过在线可视化评估脑微循环一直是不可能的。边流暗场（SDF）成像技术是一种相对较新的方法，它使用手持式探头直接接触目标组织，可直接可视化脑表面层微循环。本研究的目的是阐明使用 SDF 成像原位研究脑微循环的可行性，并评估机械通气兔的基本脑微循环参数。通过开颅术从脑表面使用 SDF 成像获得图像。成功获得了清晰的高对比度 SDF 图像。总小血管密度为 14.6+/-1.8 mm/mm(2)，总血管密度为 17.9+/-1.7 mm/mm(2)，DeBacker 评分 12.0+/-1.6 mm(-1)，微血管血流指数为 3.0+/-0.0。该方法似乎适用于动物研究，并且有可能在手术中也使用 SDF 成像，为在各种实验和临床环境下研究脑微循环提供了独特的机会。
