Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Plastic and Reconstructive Surgery, Aichi Cancer Center Hospital, Nagoya, Japan.
Nagoya J Med Sci. 2020 May;82(2):291-300. doi: 10.18999/nagjms.82.2.291.
The purpose of this study was to assess the correlation between tissue volume and blood flow of the flap in an animal model. Using animal model, tissue volume can be attenuated, and precise change of blood flow could be evaluated. We further investigate the relationship between blood flow and vascular density in the tissue. In this study, we assessed flap conductance (ml/min/mm Hg) as to evaluate the conductivity of blood flow into the flap. Japanese white rabbit was used (n = 7) for this study. The amount of blood flow of jejunal and latissimus dorsi muscle (LD) flaps was measured while removing the distal portion of the flap sequentially. Conductance at each time was calculated from blood pressure and blood flow volume. The tissue volume at each time was also measured. The correlation between conductance and volume was analyzed using a linear mixed model. Immunohistochemical evaluation of microvessel densities (MVD) in these tissues was also performed for CD31/PECAM1 positive area. Conductance and tissue volume were significantly correlated in both jejunal and LD flaps. As the volume increases by 1 cm, the conductance increased significantly by 0.012 ml/min/mm Hg in jejunum, and by 0.0047 ml/min/mm Hg in LD. Mean MVD was 1.15 ± 0.52% in the jejunum and 0.37 ± 0.29% in the LD muscle. In this study, we revealed that flap conductance is proportional to volume and proportional constant is different between the type of tissue. It suggests that the difference of MVD creates the unique conductance of each tissue.
本研究旨在评估动物模型中皮瓣组织体积与血流量之间的相关性。使用动物模型，可以减弱组织体积，并可以精确评估血流量的变化。我们进一步研究了组织中血流量与血管密度之间的关系。在这项研究中，我们评估了皮瓣导纳（ml/min/mm Hg），以评估血流进入皮瓣的导纳能力。本研究使用日本大白兔（n = 7）。在依次切除皮瓣的远端部分的同时，测量空肠和背阔肌（LD）皮瓣的血流量。在每个时间点，根据血压和血流量计算导纳。同时测量每个时间点的组织体积。使用线性混合模型分析导纳与体积之间的相关性。还对这些组织中微血管密度（MVD）进行了 CD31/PECAM1 阳性面积的免疫组织化学评估。在空肠和 LD 皮瓣中，导纳和组织体积均呈显著相关性。当体积增加 1cm 时，空肠的导纳显著增加 0.012ml/min/mm Hg，LD 增加 0.0047ml/min/mm Hg。空肠的平均 MVD 为 1.15 ± 0.52%，LD 肌肉为 0.37 ± 0.29%。在这项研究中，我们揭示了皮瓣导纳与体积成正比，并且组织类型之间的比例常数不同。这表明 MVD 的差异导致了每种组织独特的导纳。
