Rodríguez Clara I, Simón Carlos
Valencia Stem Cell Bank, Centro de Investigación Principe Felipe, Avda. Autopista del Saler, Valencia, Spain.
Regen Med. 2006 Jan;1(1):103-9. doi: 10.2217/17460751.1.1.103.
Human embryonic stem cells (hESC) are capable of proliferating indefinitely in an undifferentiated state and are pluripotent, being able to differentiate into most cell types under the correct conditions. Since the establishment of the first hESC line in 1998, the hope has existed that these cells could constitute an unlimited cell source for replacement therapy in the treatment of various diseases and disabilities. However, there is opposition and concern within society towards hESC derivation. The purpose of this article is to introduce the medical and scientific issues surrounding hESC derivation for clinical use concerning the source for this research (human embryos donated from in vitro fertilization procedures), and the methodologies implicated in feeder-free, xeno-free derivation that will allow potential clinical applications.
人类胚胎干细胞（hESC）能够在未分化状态下无限增殖，并且具有多能性，即在正确的条件下能够分化为大多数细胞类型。自1998年首个hESC系建立以来，人们一直希望这些细胞能够成为治疗各种疾病和残疾的替代疗法的无限细胞来源。然而，社会上存在对hESC来源的反对和担忧。本文的目的是介绍围绕hESC来源用于临床的医学和科学问题，涉及该研究的来源（体外受精程序捐赠的人类胚胎）以及无饲养层、无异种的来源所涉及的方法，这些方法将允许潜在的临床应用。
