López Ana C, Alippi Adriana M
Centro de Investigaciones de Fitopatología, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, calle 60 y 118 s/n, 1900 La Plata, Argentina.
Int J Food Microbiol. 2007 Jun 30;117(2):175-84. doi: 10.1016/j.ijfoodmicro.2007.03.007. Epub 2007 Mar 30.
Of 433 honey samples collected in Argentina, 114 (27%) yielded Bacillus cereus isolates (27%) and 60 (14%) yielded other species of Bacillus. The Argentinian B. cereus isolates were compared with isolates recovered from honey from other countries (n=16) and with strain ATCC 11718. The 133 B. cereus isolates and the ATCC strain were characterized using rep-PCR fingerprinting with primers BOX, REP and ERIC, restriction fragment length polymorphism analysis of a 16S rRNA gene fragment (16S rRNA PCR/RFLP), and morphological and biochemical tests. There was a high degree of diversity, both phenotypic and genotypic among the isolates of B. cereus. Our results demonstrate the usefulness of rep-PCR genomic fingerprinting for characterizing populations of B. cereus. According to our knowledge, this is the first report of phenotypic and genotypic characterization of B. cereus isolates from honey.
在阿根廷采集的433份蜂蜜样本中，114份（27%）分离出蜡样芽孢杆菌（27%），60份（14%）分离出其他芽孢杆菌属物种。将阿根廷的蜡样芽孢杆菌分离株与从其他国家蜂蜜中分离出的菌株（n = 16）以及ATCC 11718菌株进行比较。使用BOX、REP和ERIC引物的重复序列聚合酶链反应指纹图谱、16S rRNA基因片段的限制性片段长度多态性分析（16S rRNA PCR/RFLP）以及形态学和生化试验，对133株蜡样芽孢杆菌分离株和ATCC菌株进行了鉴定。蜡样芽孢杆菌分离株在表型和基因型上都存在高度多样性。我们的结果证明了重复序列聚合酶链反应基因组指纹图谱在鉴定蜡样芽孢杆菌群体方面的实用性。据我们所知，这是关于蜂蜜中蜡样芽孢杆菌分离株表型和基因型鉴定的首次报告。
