Department of Basic Sciences, College of Forestry, Dr YSP University of Horticulture & Forestry Nauni, Solan, 173230, Himachal Pradesh, India.
Department of Entomology, College of Horticulture, Dr YSP University of Horticulture & Forestry Nauni, Solan, 173230, Himachal Pradesh, India.
Arch Microbiol. 2021 Oct;203(8):5029-5041. doi: 10.1007/s00203-021-02489-y. Epub 2021 Jul 21.
This paper presents a pioneer study on the microbial diversity and antibacterial potential of hive bees (Apis cerana and A. mellifera) honey collected from Himachal Pradesh. In total, 26 bacteria (14 from A. cerana and 12 from A. mellifera) but no fungal isolate were recovered. Bee species and locations comparison in terms of bacterial load (log CFU/g) revealed maximum loads of 3.74 and 3.99 in the honey from A. cerana and Mandi location, respectively. The most prevalent strains (HC3, HC5, HC6, HC8 and HM2) were identified (16S rRNA ribotyping) as Staphylococcus haemolyticus (MT742636), "Bacillus subtilis subsp. stecoris" (MT742637), Bacillus safensis subsp. safensis (MT742638), "Bacillus zanthoxyli" (MT742639) and Bacillus safensis subsp. safensis (MT938911). The apiary honey displayed good to excellent inhibitory activity against Escherichia coli ATCC1041 whereas, fair to good against Bacillus subtilis ATCC6633, Pseudomonas aeruginosa ATCC10662, Salmonella typhi NCTC786 and Klebsiella pneumoniae ATCC13883, highlighting its use as a therapeutic agent. Furthermore, it can be effective in minimizing numerous side effects associated with the consumption of synthetic drugs for treating bacterial infections thereby signifying the role of honey as a healthier substitute for synthetic drugs.
本文对来自喜马偕尔邦的中华蜜蜂（Apis cerana 和 A. mellifera）蜂蜜中的微生物多样性和抗菌潜力进行了开创性研究。共回收了 26 株细菌（14 株来自 A. cerana，12 株来自 A. mellifera），但未分离出真菌。根据细菌负荷（log CFU/g）比较蜜蜂种类和地点，发现 A. cerana 和曼迪（Mandi）产的蜂蜜中的细菌负荷最高，分别为 3.74 和 3.99。最常见的菌株（HC3、HC5、HC6、HC8 和 HM2）通过 16S rRNA 核糖体分型鉴定为溶血葡萄球菌（MT742636）、“枯草芽孢杆菌亚种 steccoris”（MT742637）、巴氏芽孢杆菌 safensis subsp. safensis（MT742638）、“黄杆菌”（MT742639）和巴氏芽孢杆菌 safensis subsp. safensis（MT938911）。养蜂场的蜂蜜对大肠杆菌 ATCC1041 显示出良好至优异的抑制活性，而对枯草芽孢杆菌 ATCC6633、铜绿假单胞菌 ATCC10662、伤寒沙门氏菌 NCTC786 和肺炎克雷伯菌 ATCC13883 则显示出良好至优异的抑制活性，这突出了其作为治疗剂的用途。此外，它可以有效减少与使用合成药物治疗细菌感染相关的许多副作用，从而凸显了蜂蜜作为合成药物更健康替代品的作用。
