Harinisri Karthikeyan, Prathiviraj Ragothaman, Thamarai Selvi Balasubramanian
Department of Microbiology, Sri Ramakrishna College of Arts & Science for Women, Coimbatore, 641044, Tamil Nadu, India.
Department of Microbiology, Pondicherry University, Puducherry, 605014, India.
Biotechnol Notes. 2024 Nov 15;5:151-164. doi: 10.1016/j.biotno.2024.11.003. eCollection 2024.
This research evaluated a bacterial strain, (S55), isolated from the soil for its bioflocculant production capabilities. The strain was identified through morphological and 16s rRNA gene sequencing. The optimization of culture conditions, using One-factor-at-a-time method, significantly enhanced bioflocculant production with glucose and urea. FTIR analysis showed the presence of hydroxyl, amine and carboxylate functional groups, with polysaccharides as predominant components. Scanning electron microscopy and X-ray diffraction confirmed the crystalline nature of the bioflocculant. The strain studied showed potential in treating household wastewater and was effective at removing dyes, suggesting alternatives for wastewater management.
本研究评估了从土壤中分离出的一株细菌菌株(S55)的生物絮凝剂生产能力。通过形态学和16s rRNA基因测序对该菌株进行了鉴定。采用一次单因素法对培养条件进行优化,显著提高了葡萄糖和尿素的生物絮凝剂产量。傅里叶变换红外光谱分析表明存在羟基、胺基和羧酸盐官能团,主要成分是多糖。扫描电子显微镜和X射线衍射证实了生物絮凝剂的晶体性质。所研究的菌株在处理生活污水方面显示出潜力,并且在去除染料方面有效,为废水管理提供了替代方案。