Sherwood Nancy M, Adams Bruce A, Isaac Emma R, Wu Sheng, Fradinger Erica A
Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada.
Peptides. 2007 Sep;28(9):1680-7. doi: 10.1016/j.peptides.2007.03.008. Epub 2007 Mar 24.
One approach to understanding the role of PACAP in vivo is to knockdown the translation of PACAP mRNA to protein or to knock out the PACAP gene by targeted disruption. In this paper, we review the effect of PACAP knockdown with morpholinos on early brain development in zebrafish. Also reviewed is the role of PACAP at several stages of reproduction as assessed in mice with a disrupted PACAP gene. New data are presented to analyze PACAP's action in energy homeostasis (body mass, food intake, endocrine parameters) using female PACAP-null mice. The evidence suggests PACAP is important for brain development in zebrafish and is required for normal reproduction, but not for body mass or food intake in mice maintained near thermoneutrality.
理解垂体腺苷酸环化酶激活肽（PACAP）在体内作用的一种方法是抑制PACAP信使核糖核酸（mRNA）向蛋白质的翻译，或通过靶向破坏来敲除PACAP基因。在本文中，我们综述了用吗啉代寡核苷酸抑制PACAP对斑马鱼早期脑发育的影响。同时也综述了在PACAP基因被破坏的小鼠中评估的PACAP在几个生殖阶段的作用。本文还展示了新的数据，以利用雌性PACAP基因缺失小鼠来分析PACAP在能量平衡（体重、食物摄入量、内分泌参数）方面的作用。证据表明，PACAP对斑马鱼的脑发育很重要，是正常生殖所必需的，但对处于接近热中性状态的小鼠的体重或食物摄入量并非必需。
