Matsuda Kouhei, Maruyama Keisuke, Miura Tohru, Uchiyama Minoru, Shioda Seiji
Department of Biology, Faculty of Science, Toyama University, Toyama, Toyama 930-8555, Japan.
Neurosci Lett. 2005 Sep 23;386(1):9-13. doi: 10.1016/j.neulet.2005.05.053.
Our recent research has indicated that intracerebroventricular (ICV) administration of pituitary adenylate cyclase-activating polypeptide (PACAP) suppresses food intake in the goldfish. We therefore examined feeding- and fasting-induced changes in the expression of mRNAs for PACAP and its receptors in the goldfish brain, and the effect of ICV administration of synthetic PACAP on locomotor activity in the goldfish. Semiquantitative analysis revealed that the expression of mRNAs for PACAP and the PAC1 receptor was significantly increased by excessive feeding (daily food supply at >or=5% of the body weight [BW]) for 7 days. ICV administration of PACAP at 20 pmol/g BW induced a significant decrease in locomotor activity during the 60-min post-treatment observation period. These results suggest that PACAP may have an anorexigenic action via hypomotility in goldfish.
我们最近的研究表明，脑室内（ICV）注射垂体腺苷酸环化酶激活多肽（PACAP）可抑制金鱼的食物摄入。因此，我们研究了喂食和禁食诱导的金鱼脑中PACAP及其受体mRNA表达的变化，以及脑室内注射合成PACAP对金鱼运动活动的影响。半定量分析显示，连续7天过度喂食（每日食物供应量≥体重[BW]的5%）可显著增加PACAP和PAC1受体mRNA的表达。以20 pmol/g BW的剂量脑室内注射PACAP可导致治疗后60分钟观察期内运动活动显著减少。这些结果表明，PACAP可能通过降低金鱼的运动活性发挥厌食作用。
