Clinical validation of a new blood collection tube for the accuracy of total homocysteine measurement by different methods.

作者信息

Bissé Emmanuel, Epting Thomas, Kögel Gabriele, Obeid Rima, Gempel Klaus, Huzly Daniela, Fuhrmann Klaus, Rolinski Boris, Herrmann Wolfgang, Wieland Heinrich

机构信息

Department of Clinical Chemistry, University Medical Center Freiburg, Hugstetterstrasse 55, D-79106 Freiburg, Germany.

出版信息

Clin Biochem. 2007 Jun;40(9-10):739-43. doi: 10.1016/j.clinbiochem.2007.03.005. Epub 2007 Mar 20.

OBJECTIVE

To evaluate the clinical use of Homocysteine-Primavette, a new blood collection medium for total homocysteine (tHcy) assay.

METHODS

The agreement between baseline tHcy and tHcy in stabilized samples (40 h) was assessed for FPIA, HPLC, GC-MS, LC-MS, and ICL.

RESULTS

tHcy concentrations in whole blood were stable for 40 h in Hcy-Primavette tubes.

CONCLUSION

Primavette tubes are a good alternative for the accurate tHcy measurement and no readjustment of reference intervals is needed.