Góes J C, Figueiró S D, Oliveira A M, Macedo A A M, Silva C C, Ricardo N M P S, Sombra A S B
Laboratório de Telecomunicações e Ciência dos Materiais (LOCEM), Departamento de Física, Universidade Federal do Ceará, Caixa Postal 6030, CEP 60455-760, Fortaleza, Ceará, Brazil.
Acta Biomater. 2007 Sep;3(5):773-8. doi: 10.1016/j.actbio.2007.02.008. Epub 2007 Apr 30.
The present study focuses on apatite coating on collagen films, with various different densities of carboxyl groups, using an alternate soaking process. Anionic collagen (AC), which has different densities of carboxylic groups compared to native collagen (NC), was obtained by hydrolysis of carboxyamides of asparagine and glutamine residues. From X-ray diffraction analysis, apatite was found to be coated on AC and NC films. Peaks ascribed to apatite were observed at 26 degrees and 32 degrees in the diffraction patterns of hydroxyapatite crystals. The amount of apatite coated on both AC and NC collagen films continued to increase up to 100 reaction cycles. However, there is a significant difference in apatite coating between the two films. The amount of apatite formed on the surface of AC film increased 1.24 times faster than on NC film. The scanning electron photomicrograph images of the mineralized NC and the AC film coatings formed after 100cycles show that regular porous apatite coating had formed within the collagen fibrils. These results suggest that the higher content of carboxyl groups in AC plays an effective role in the heterogeneous nucleation of apatite in the body environment.
本研究聚焦于使用交替浸泡工艺在具有不同羧基密度的胶原膜上进行磷灰石涂层。通过天冬酰胺和谷氨酰胺残基的羧酰胺水解获得了与天然胶原（NC）相比具有不同羧基密度的阴离子胶原（AC）。通过X射线衍射分析发现，磷灰石被包覆在AC膜和NC膜上。在羟基磷灰石晶体的衍射图谱中，在26度和32度处观察到归属于磷灰石的峰。在AC和NC胶原膜上包覆的磷灰石量在100个反应循环之前持续增加。然而，两种膜之间的磷灰石涂层存在显著差异。AC膜表面形成的磷灰石量比NC膜增加快1.24倍。100个循环后形成的矿化NC和AC膜涂层的扫描电子显微照片显示，在胶原纤维内形成了规则的多孔磷灰石涂层。这些结果表明，AC中较高的羧基含量在体内环境中磷灰石的异质成核过程中发挥了有效作用。
