Brown J M, Stastny J J, Beattie C W, Das Gupta T K
Specialized Center for Cancer Research and Education, University of Illinois College of Medicine, Chicago 60612.
Anticancer Res. 1991 Jul-Aug;11(4):1565-70.
A membrane protein of Mr 102,000 Da (p102) was detected in 51 out of 53 human sarcomas, using a murine IgG1 monoclonal antibody 23-26. Antigen expression in sarcoma histologic subtypes appeared dependent on the amount of fibrous tissue matrix present in the original specimen. High levels of p102 antigen were also found in all sarcoma, carcinoma cell lines and neonatal skin fibroblasts tested. Low levels of p102 were also expressed in membranes from some specimens of melanoma, lung and colorectal carcinoma, first trimester fetus, fat, lung and liver while skin specimens expressed slightly higher levels of antigen. Lectin affinity absorption indicated p102 was mannose containing glycoprotein, isoelectric point (pI) 4.7 and affinity constant of 2.3 x 10(9) M-1, with 5.9 x 10(5) binding sites per cultured human HT-1080 fibrosarcoma cell. The overexpression of p102 in sarcomas and other neoplastic tissues suggests that this protein may be associated with the neoplastic state.
利用鼠IgG1单克隆抗体23 - 26，在53例人类肉瘤中的51例中检测到一种分子量为102,000道尔顿的膜蛋白（p102）。肉瘤组织学亚型中的抗原表达似乎取决于原始标本中纤维组织基质的含量。在所有测试的肉瘤、癌细胞系和新生儿皮肤成纤维细胞中也发现了高水平的p102抗原。在一些黑色素瘤、肺癌和结直肠癌标本、孕早期胎儿、脂肪、肺和肝脏的膜中也有低水平的p102表达，而皮肤标本表达的抗原水平略高。凝集素亲和吸附表明p102是含甘露糖的糖蛋白，等电点（pI）为4.7，亲和常数为2.3×10⁹ M⁻¹，每个培养的人HT - 1080纤维肉瘤细胞有5.9×10⁵个结合位点。p102在肉瘤和其他肿瘤组织中的过表达表明该蛋白可能与肿瘤状态有关。
