Strother D R, Parham D M, Houghton P J
Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, TN 38101-0318.
Arch Pathol Lab Med. 1990 Jun;114(6):593-6.
The monoclonal antibody 5.1 H11 recognizes an antigen on human fetal muscle and on rhabdomyosarcoma cell lines and xenografts that has been shown to be homologous to the neural cell adhesion molecule. To evaluate its range of expression, we used immunoperoxidase staining of fresh frozen-tissue sections to determine monoclonal antibody 5.1 H11 reactivity in normal and neoplastic tissue. Among normal tissue specimens, intense antibody staining was seen in brain and peripheral nerve, and weaker staining in ganglionic elements of colon. In addition to 26 of 29 rhabdomyosarcoma specimens, 5.1 H11 antibody showed reactivity to 9 of 10 Wilms' tumors, 6 of 6 neural tumors, and 4 of 4 gliomas, and with single specimens of ectomesenchymoma, clear-cell sarcoma of kidney, undifferentiated sarcoma of liver, ovarian fibroma, and neurofibroma. We conclude that the monoclonal antibody 5.1 H11 recognizes an antigen present not only on fetal muscle but on normal brain and nerve as well. In addition to rhabdomyosarcoma, a variety of other tumors, most of which have been previously shown to express neural cell adhesion molecule, also appear to express the antigen recognized by 5.1 H11. Our results thus offer additional confirmatory evidence that an epitope of neural cell adhesion molecule is the antigen for 5.1 H11.
单克隆抗体5.1 H11可识别一种存在于人类胎儿肌肉、横纹肌肉瘤细胞系及异种移植物上的抗原，该抗原已被证明与神经细胞黏附分子具有同源性。为评估其表达范围，我们采用免疫过氧化物酶法对新鲜冰冻组织切片进行染色，以确定单克隆抗体5.1 H11在正常组织和肿瘤组织中的反应性。在正常组织标本中，脑和周围神经可见强烈的抗体染色，结肠神经节成分染色较弱。除29例横纹肌肉瘤标本中的26例外，5.1 H11抗体对10例肾母细胞瘤中的9例、6例神经肿瘤中的6例、4例神经胶质瘤中的4例有反应，且对间叶瘤、肾透明细胞肉瘤、肝未分化肉瘤、卵巢纤维瘤和神经纤维瘤的单个标本也有反应。我们得出结论，单克隆抗体5.1 H11识别的抗原不仅存在于胎儿肌肉上，也存在于正常脑和神经上。除横纹肌肉瘤外，多种其他肿瘤（其中大多数先前已证明表达神经细胞黏附分子）似乎也表达5.1 H11识别的抗原。因此，我们的结果提供了额外的确证证据，即神经细胞黏附分子的一个表位是5.1 H11的抗原。
