Wang D-M, Yao Y-J
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Can J Microbiol. 2005 Feb;51(2):113-21. doi: 10.1139/w04-118.
Intrastrain internal transcribed spacer (ITS) heterogeneity is first reported from Ganoderma, a fungal genus within Basidiomycetes. ITS amplification products from 4 strains, representing 4 Ganoderma species, were cloned and sequenced. Two to five different ITS types were found within a single strain. The clone sequences were analyzed along with other sequences from Ganoderma retrieved from GenBank. The results show that sequence variation within strains varies considerably with species and the heterogeneity may occur in the 3 parts (ITS1, ITS2, and 5.8S) of the ITS region.
首次在担子菌纲真菌灵芝属中报道了菌株内转录间隔区（ITS）的异质性。对代表4种灵芝的4个菌株的ITS扩增产物进行了克隆和测序。在单个菌株中发现了2至5种不同的ITS类型。将克隆序列与从GenBank检索到的灵芝其他序列一起进行了分析。结果表明，菌株内的序列变异因物种而异，并且异质性可能发生在ITS区域的3个部分（ITS1、ITS2和5.8S）。
