通过抑制光泽精的化学发光对胆红素进行定量测定。

Quantitative determination of bilirubin by inhibition of chemiluminescence from lucigenin.

作者信息

Lee Hyun Sook, Karim Mohammad Mainul, Alam Seikh Mafiz, Lee Sang Hak

机构信息

Department of Sensor and Display Engineering, Kyungpook National University, Taegu 702-701, Republic of Korea.

出版信息

Luminescence. 2007 Jul-Aug;22(4):331-7. doi: 10.1002/bio.967.

DOI:10.1002/bio.967

PMID:17471465

Abstract

Bilirubin is a metabolic breakdown product of blood haem, of great biological and diagnostic importance. A new chemiluminescence (CL) method has been developed for the quantification of bilirubin. The method is combined with the flow injection analysis (FIA) technique and based on the inhibition effect of bilirubin on the CL from the lucigenin-hydrogen peroxide system in an alkaline medium. Under the optimum conditions, the decreased CL intensity was proportional to the concentration of bilirubin, in the range 0.0585-58.47 microg/mL. The detection limit estimated from the calibration graph was about 7.8826 ng/mL. The relative standard deviation (RSD) of 10 parallel measurements (1 x 10(-4) mol/L bilirubin) was 2.5%. Recoveries of bilirubin were found to fall in the range 94-97.5% using control sera. The method is interference-free, fast and easy to carry out.

摘要

胆红素是血液中血红素的代谢分解产物，具有重要的生物学和诊断意义。已开发出一种用于定量胆红素的新型化学发光（CL）方法。该方法与流动注射分析（FIA）技术相结合，基于胆红素在碱性介质中对光泽精 - 过氧化氢体系化学发光的抑制作用。在最佳条件下，化学发光强度的降低与胆红素浓度成正比，浓度范围为0.0585 - 58.47μg/mL。根据校准曲线估算的检测限约为7.8826 ng/mL。10次平行测量（1×10⁻⁴mol/L胆红素）的相对标准偏差（RSD）为2.5%。使用对照血清时，胆红素的回收率在94 - 97.5%范围内。该方法无干扰、快速且易于实施。