流动注射分析结合鲁米诺-六氰合铁(III)化学发光检测法测定多巴胺

Determination of dopamine by flow-injection analysis coupled with luminol-hexacyanoferrate(III) chemiluminescence detection.

作者信息

Nalewajko Edyta, Bort Ramírez Rosa, Kojło Anatol

机构信息

Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.

出版信息

J Pharm Biomed Anal. 2004 Sep 21;36(1):219-23. doi: 10.1016/j.jpba.2004.05.009.

DOI:10.1016/j.jpba.2004.05.009

PMID:15351069

Abstract

A novel flow-injection method (FIA) for the determination of dopamine based on the inhibition of the intensity of chemiluminescence (CL) from luminol-hexacyanoferrate(III) system in basic medium is described. The present method allows the determination of dopamine over the range 30-100 microg l(-1) and 400-3000 microg l(-1). The relative standard deviation is 2.32% for 70 microg l(-1) dopamine and 1.22% for 1500 microg l(-1) dopamine (n = 20). The detection limit is 5 microg l(-1) with the sampling rate of 135 samples h(-1). This method has been applied for the determination of dopamine in commercial pharmaceutical injection samples. The results obtained by this method agreed with those by the official method.

摘要

描述了一种基于碱性介质中鲁米诺 - 铁氰化铁（III）体系化学发光（CL）强度抑制的新型流动注射法（FIA）用于测定多巴胺。本方法可测定30 - 100 μg l⁻¹和400 - 3000 μg l⁻¹范围内的多巴胺。对于70 μg l⁻¹的多巴胺，相对标准偏差为2.32%，对于1500 μg l⁻¹的多巴胺，相对标准偏差为1.22%（n = 20）。检测限为5 μg l⁻¹，采样速率为135个样品 h⁻¹。该方法已应用于商业药用注射剂样品中多巴胺的测定。该方法得到的结果与官方方法的结果一致。