Ben Yahia R, Lichnovská R, Janusová L, Kuzmina G, Karpísek M, Kollár P, Petrek J
Ustav fyziologie Lékarské fakulty UP, Olomouc.
Vnitr Lek. 2007 Jan;53(1):9, 11-7.
Endocrinal products of adipocytes (PPARgamma, A-FABP, E-FABP, leptin, adiponectin and others) modulate insulin tissue sensitivity enabling them to participate in the ethiopathogenesis of diabetes mellitus type 2 (DM2T). Persons with DM2T are characterised by typical changes in lipid spectrum (lower HDL-cholesterol and higherTAG) and in the endocrinal function of subcutaneous adipose tissue; adipocytes produce more PPARgamma, A-FABP and E-FABP and less adiponectin.
To measure chosen markers of metabolic syndrome (MS) in serum and in abdominal subcutaneous adipose tissue in healthy persons and patients with DMT2, to determine basic statistical characteristics of investigated parameters and to discus their role in the genesis and progress of the MS.
Samples of blood and abdominal subcutaneous adipose tissue were collected from each participant (healthy: 7 men and 8 women; diabetics: 18 men, 11 women) to investigate the levels of HDL, TAG, insulin, C-peptide, glycaemia and the concentrations of A-FABP, E-FABP, leptin, adiponectin, resistin, PPARgamma and TNFalpha.
In most cases the average concentration of investigated parameters in serum was higher in persons with DM 2 regardless of gender. Lower values were only found for HDL and adiponectin. The same situation prevailed in the subcutaneous adipose tissue. Values of most other parameters (A-FABP, E-FABP, and PPARgamma) were also higher in patients. The values of measured parameters not only differed in healthy and in sick persons but depended on gender. The increase/decrease in concrete parameters was greater in diabetic women than diabetic men.
Higher concentrations of A-FABP, E-FABP in serum and in subcutaneous adipose tissue in diabetic persons also higher concentrations of PPARgamma in subcutaneous adipose tissue suggest that these investigated parameters are closely associated with obesity and MS. We can assume that in the near future these parameters will be used in clinical work for diagnosis of this syndrome.
脂肪细胞的内分泌产物（过氧化物酶体增殖物激活受体γ、脂肪型脂肪酸结合蛋白A、脂肪型脂肪酸结合蛋白E、瘦素、脂联素等）调节胰岛素组织敏感性，使其参与2型糖尿病（DM2T）的发病机制。DM2T患者的特征是血脂谱（高密度脂蛋白胆固醇降低和甘油三酯升高）以及皮下脂肪组织内分泌功能的典型变化；脂肪细胞产生更多的过氧化物酶体增殖物激活受体γ、脂肪型脂肪酸结合蛋白A和脂肪型脂肪酸结合蛋白E，而脂联素减少。
测量健康人和DM2T患者血清及腹部皮下脂肪组织中选定的代谢综合征（MS）标志物，确定研究参数的基本统计特征，并探讨它们在MS发生和发展中的作用。
从每位参与者（健康人：7名男性和8名女性；糖尿病患者：18名男性，11名女性）采集血液和腹部皮下脂肪组织样本，以研究高密度脂蛋白、甘油三酯、胰岛素、C肽、血糖水平以及脂肪型脂肪酸结合蛋白A、脂肪型脂肪酸结合蛋白E、瘦素、脂联素、抵抗素、过氧化物酶体增殖物激活受体γ和肿瘤坏死因子α的浓度。
在大多数情况下，无论性别，DM2患者血清中研究参数的平均浓度较高。仅高密度脂蛋白和脂联素的值较低。皮下脂肪组织中也存在同样的情况。大多数其他参数（脂肪型脂肪酸结合蛋白A、脂肪型脂肪酸结合蛋白E和过氧化物酶体增殖物激活受体γ）的值在患者中也较高。测量参数的值不仅在健康人和患者中不同，还取决于性别。糖尿病女性具体参数的增加/减少幅度大于糖尿病男性。
糖尿病患者血清和皮下脂肪组织中脂肪型脂肪酸结合蛋白A、脂肪型脂肪酸结合蛋白E浓度较高，皮下脂肪组织中过氧化物酶体增殖物激活受体γ浓度也较高，这表明这些研究参数与肥胖和MS密切相关。我们可以假设在不久的将来，这些参数将用于临床工作中该综合征的诊断。
