Nielsen Ninna Bo, Højbjerre Lise, Sonne Mette P, Alibegovic Amra C, Vaag Allan, Dela Flemming, Stallknecht Bente
Department of Biomedical Sciences, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Regul Pept. 2009 Jun 5;155(1-3):39-45. doi: 10.1016/j.regpep.2009.04.010. Epub 2009 Apr 17.
Adipokines play important regulatory roles in the pathophysiology of obesity and insulin resistance. We measured plasma and interstitial concentrations of the adipokines adiponectin, resistin, leptin, monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) in subcutaneous, abdominal and femoral adipose tissue using calibrated, large-pore microdialysis technique in 8 healthy, lean men on 2 experimental days. The interstitial leptin concentration was 2.5-fold higher in subcutaneous, femoral than abdominal adipose tissue (P<0.05), but no regional differences were found for the remaining adipokines (P>0.05). Adiponectin and leptin concentrations were higher in plasma than subcutaneous adipose tissue (approximately 25-fold and approximately 2-fold, respectively, P<0.05), whereas MCP-1, IL-6 and IL-8 concentrations were higher in subcutaneous adipose tissue than plasma (approximately 100-fold, approximately 200-fold and approximately 1000-fold, respectively, P<0.05). Resistin concentrations did not differ significantly between compartments. Adipose tissue blood flow (ATBF) showed no regional difference (P>0.05). The intra- and inter-subject variations of all investigated adipokines as well as of ATBF were substantial (coefficient of variation: 4-177%). In conclusion, interstitial leptin concentrations are approximately 2.5-fold higher in subcutaneous, femoral than abdominal adipose tissue, which might be a potential mechanism behind the health-benefits of "pear-shape". Furthermore, subcutaneous adipose tissue has a marked production of pro-inflammatory adipokines.
脂肪因子在肥胖和胰岛素抵抗的病理生理学中发挥着重要的调节作用。我们在8名健康、体型偏瘦的男性受试者的两个实验日中，采用校准的大孔径微透析技术，测量了皮下、腹部和股部脂肪组织中脂联素、抵抗素、瘦素、单核细胞趋化蛋白-1（MCP-1）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）等脂肪因子的血浆和组织间液浓度。皮下和股部脂肪组织中的组织间液瘦素浓度比腹部脂肪组织高2.5倍（P<0.05），但其余脂肪因子未发现区域差异（P>0.05）。血浆中的脂联素和瘦素浓度高于皮下脂肪组织（分别约为25倍和约2倍，P<0.05），而皮下脂肪组织中的MCP-1、IL-6和IL-8浓度高于血浆（分别约为100倍、约200倍和约1000倍，P<0.05）。各部位间抵抗素浓度无显著差异。脂肪组织血流量（ATBF）未显示出区域差异（P>0.05）。所有研究的脂肪因子以及ATBF在受试者内和受试者间的变异都很大（变异系数：4-177%）。总之，皮下和股部脂肪组织中的组织间液瘦素浓度比腹部脂肪组织高约2.5倍，这可能是“梨形身材”有益健康的潜在机制。此外，皮下脂肪组织会大量产生促炎性脂肪因子。
