多药耐药相关蛋白9（ABCC12）存在于小鼠和公猪精子中。
Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm.
作者信息
Ono Nobuhito, Van der Heijden Ingrid, Scheffer George L, Van de Wetering Koen, Van Deemter Elizabeth, De Haas Marcel, Boerke Arjan, Gadella Bart M, De Rooij Dirk G, Neefjes Jacques J, Groothuis Tom A M, Oomen Lauran, Brocks Lenny, Ishikawa Toshihisa, Borst Piet
机构信息
Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
出版信息
Biochem J. 2007 Aug 15;406(1):31-40. doi: 10.1042/BJ20070292.
The human and murine genes for MRP9 (multidrug resistance-associated protein 9; ABCC12) yield many alternatively spliced RNAs. Using a panel of monoclonal antibodies, we detected full-length Mrp9 only in testicular germ cells and mouse sperm; we obtained no evidence for the existence of the truncated 100 kDa MRP9 protein reported previously. In contrast with other MRPs, neither murine Mrp9 nor the human MRP9 produced in MRP9-transfected HEK-293 cells (human embryonic kidney cells) appears to contain N-linked carbohydrates. In mouse and boar sperm, Mrp9 localizes to the midpiece, a structure containing all sperm mitochondria. However, immunolocalization microscopy and cell fractionation studies with transfected HEK-293 cells and mouse testis show that MRP9/Mrp9 does not localize to mitochondria. In HEK-293 cells, it is predominantly localized in the endoplasmic reticulum. We have been unable to demonstrate transport by MRP9 of substrates transported by other MRPs, such as drug conjugates and other organic anions.
多药耐药相关蛋白9（MRP9；ABCC12）的人类和小鼠基因产生许多可变剪接的RNA。我们使用一组单克隆抗体，仅在睾丸生殖细胞和小鼠精子中检测到全长Mrp9；我们没有获得证据证明先前报道的截短型100 kDa MRP9蛋白的存在。与其他MRP不同，在MRP9转染的HEK - 293细胞（人胚肾细胞）中产生的小鼠Mrp9和人类MRP9似乎都不含有N - 连接碳水化合物。在小鼠和猪的精子中，Mrp9定位于中段，这是一个包含所有精子线粒体的结构。然而，对转染的HEK - 293细胞和小鼠睾丸进行的免疫定位显微镜检查和细胞分级分离研究表明，MRP9/Mrp9并不定位于线粒体。在HEK - 293细胞中，它主要定位于内质网。我们未能证明MRP9能转运其他MRP所转运的底物，如药物缀合物和其他有机阴离子。
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