Hamano Hiroki, Yoshinaga Koji, Eta Runa, Emori Yutaka, Kawasaki Daisuke, Iino Yuka, Sawada Miwa, Kuroda Hiroyuki, Takei Mineo
Central Research Laboratories, Zeria Pharmaceutical Co., Ltd., Saitama, Japan.
Biofactors. 2006;28(3-4):185-93. doi: 10.1002/biof.5520280305.
The effect of polaprezinc, a chelate compound consisting of zinc ion and L-carnosine, on abnormalities of taste sensation induced by feeding a zinc-deficient diet to rats was examined by using the two-bottle preference test (quinine hydrochloride as a bitter taste and sodium chloride as a salty taste). Rats were fed either a zinc-deficient or a zinc-sufficient diet. The zinc-deficient diet increased the preference for both taste solutions, while polaprezinc (at doses of 3 and 10 mg/kg) restored the altered taste preferences. We also evaluated the proliferation of taste bud cells using 5-bromo-2'-deoxyuridine (BrdU). The BrdU incorporation into taste bud cells was significantly reduced in rats fed a zinc-deficient diet compared with rats fed a zinc-sufficient diet (from 50.8% to 45.0%, p<0.05) and this reduction was reversed by polaprezinc at doses of 1, 3, and 10 mg/kg, increasing to 50.2%, 53.5%, and 52.5%, respectively. These findings indicate that zinc deficiency induces the delayed of proliferation of taste bud cells, while polaprezinc improves cell proliferation. In conclusion, polaprezinc had a therapeutic effect in a rat model of abnormal taste sensation. Its mechanism of action was suggested to involve improvement of the decrease in taste bud cell proliferation caused by zinc deficiency.
通过双瓶偏好试验（以盐酸奎宁作为苦味剂，氯化钠作为咸味剂），研究了由锌离子和L-肌肽组成的螯合物聚普瑞锌对给大鼠喂食缺锌饮食所诱发的味觉异常的影响。将大鼠分为缺锌组和锌充足组进行饲养。缺锌饮食增加了大鼠对两种味觉溶液的偏好，而聚普瑞锌（剂量为3和10毫克/千克）恢复了改变的味觉偏好。我们还使用5-溴-2'-脱氧尿苷（BrdU）评估了味蕾细胞的增殖情况。与喂食锌充足饮食的大鼠相比，喂食缺锌饮食的大鼠味蕾细胞中BrdU掺入率显著降低（从50.8%降至45.0%，p<0.05），而1、3和10毫克/千克剂量的聚普瑞锌可逆转这种降低，掺入率分别增加至50.2%、53.5%和52.5%。这些发现表明，锌缺乏会导致味蕾细胞增殖延迟，而聚普瑞锌可改善细胞增殖。总之，聚普瑞锌对味觉异常的大鼠模型具有治疗作用。其作用机制可能是改善锌缺乏引起的味蕾细胞增殖减少。
