Williams Jonathan P, Hilton Gillian R, Thalassinos Konstantinos, Jackson Anthony T, Scrivens James H
Department of Biological Sciences, University of Warwick, Gibbet Hill Rd., Coventry CV4 7AL, UK.
Rapid Commun Mass Spectrom. 2007;21(11):1693-704. doi: 10.1002/rcm.3008.
A rapid method for the characterisation of polyglycol esters and ethers is described which uses accurate mass desorption electrospray ionisation (DESI) quadrupole time-of-flight mass spectrometry (Q-ToFMS). The results are combined with newly developed software which aids the interpretation of product ions produced using collision-induced dissociation (CID) of selected precursor ions. The poly(ethylene glycol) (PEG) samples analysed were PEG dibenzoate, PEG monooleate, PEG butyl ether, PEG bis(2-ethyl hexanoate) and PEG diacrylate. Lithium metal was used for cationisation of the PEG oligomers since it yielded the most useful structural information compared with other group I metals. The full scan mass spectra and product ion mass spectra were all obtained in <5 s. Interpretation of the MS/MS product ion spectra, using the product ion interpretation software which incorporates previously developed fragmentation rules, was carried out in <1 s.
描述了一种用于表征聚乙二醇酯和醚的快速方法，该方法使用精确质量解吸电喷雾电离（DESI）四极杆飞行时间质谱（Q-ToFMS）。结果与新开发的软件相结合，该软件有助于解释使用选定前体离子的碰撞诱导解离（CID）产生的产物离子。分析的聚（乙二醇）（PEG）样品有聚乙二醇二苯甲酸酯、聚乙二醇单油酸酯、聚乙二醇丁醚、聚乙二醇双（2-乙基己酸酯）和聚乙二醇二丙烯酸酯。锂金属用于PEG低聚物的阳离子化，因为与其他第一主族金属相比，它产生了最有用的结构信息。全扫描质谱和产物离子质谱均在5秒内获得。使用包含先前开发的碎裂规则的产物离子解释软件对MS/MS产物离子谱进行解释的时间不到1秒。
