Kim Dae-Sung, Kim Ji Young, Kang Minkyung, Cho Myung Soo, Kim Dong-Wook
Department of Physiology, Yonsei University College of Medicine, Seodaemun-gu, Seoul, Korea.
Cell Transplant. 2007;16(2):117-23.
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the selective degeneration of dopaminergic (DA) neurons in the substantia nigra of the midbrain. Pharmacological treatment of PD has been a prevailing strategy. However, it has some limitations because its effectiveness gradually decreases and side effects develop. As an alternative, cell transplantation therapy has been tried. Although transplantation of fetal ventral mesencephalic cells looks promising for the treatment of PD in some cases, ethical and technical problems in obtaining large numbers of human fetal brain tissues also lead to difficulty in its clinical application. Our recent studies showed that a high yield of DA neurons could be derived from embryonic stem (ES) cells and they efficiently induced behavioral recovery in a PD animal model. Here we summarize methods for generation of functional DA neurons from ES cells for application to PD models.
帕金森病（PD）是一种神经退行性疾病，其特征是中脑黑质中多巴胺能（DA）神经元的选择性退化。帕金森病的药物治疗一直是一种普遍采用的策略。然而，它存在一些局限性，因为其有效性会逐渐降低且会产生副作用。作为一种替代方法，人们尝试了细胞移植疗法。尽管在某些情况下，移植胎儿腹侧中脑细胞对于帕金森病的治疗看起来很有前景，但获取大量人类胎儿脑组织存在伦理和技术问题，这也导致其临床应用困难。我们最近的研究表明，可以从胚胎干细胞（ES细胞）中高效获得多巴胺能神经元，并且它们能在帕金森病动物模型中有效诱导行为恢复。在此，我们总结了从胚胎干细胞生成功能性多巴胺能神经元以应用于帕金森病模型的方法。
