Nagasawa T, Satoh K, Abe T
Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
Int J Hematol. 1991 Apr;54(2):159-64.
Partially purified thrombopoietic factor (TPF) potentiated in vitro murine megakaryopoiesis induced by recombinant human erythropoietin (rh-Epo). High doses (2.5-10 units) of rh-Epo generated a considerable number of megakaryocytes in a dose-dependent manner in a serum-free liquid culture, whereas low doses of rh-Epo (0.5-1 units) failed to generate megakaryocytes. The addition of high doses of rh-Epo and TPF caused a significant increase of megakaryocytes in comparison with high doses of rh-Epo alone. Furthermore, low doses of rh-Epo with TPF caused generation of a small number of megakaryocytes, although TPF alone did not generate megakaryocytes. In addition, TPF enhanced the acetylcholinesterase (Ach-E) activity of megakaryocytes induced by rh-Epo. The potentiating effects of both factors, rh-Epo and thrombopoietic factor, may play an important role in thrombocytopenic states in vivo as well as in in vitro megakaryopoiesis.
部分纯化的血小板生成因子（TPF）增强了重组人促红细胞生成素（rh-Epo）诱导的体外小鼠巨核细胞生成。在无血清液体培养中，高剂量（2.5 - 10单位）的rh-Epo以剂量依赖的方式产生了相当数量的巨核细胞，而低剂量的rh-Epo（0.5 - 1单位）未能产生巨核细胞。与单独使用高剂量rh-Epo相比，添加高剂量的rh-Epo和TPF导致巨核细胞显著增加。此外，低剂量的rh-Epo与TPF一起导致产生少量巨核细胞，尽管单独的TPF不能产生巨核细胞。另外，TPF增强了rh-Epo诱导的巨核细胞的乙酰胆碱酯酶（Ach-E）活性。rh-Epo和血小板生成因子这两种因子的增强作用可能在体内血小板减少状态以及体外巨核细胞生成中都发挥重要作用。
