Sakaguchi M, Kawakita M, Matsushita J, Shibuya K, Koishihara Y, Takatsuki K
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Exp Hematol. 1987 Nov;15(10):1028-34.
Recombinant erythropoietin (Epo) was capable of stimulating murine megakaryopoiesis both in serum-containing and serum-free cultures, although a relatively high amount of Epo was necessary to provide sufficient stimulus for colony growth. This observation was further confirmed by experiments using nonadherent, nonphagocytic, and T-cell-depleted marrow cells in which Epo stimulated the growth of single megakaryocytes, as well as clusters or colonies. Total plate analysis revealed that twice as many single megakaryocytes and two-cell aggregates were generated by Epo than generated by pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). The number of colonies with four or more cells formed by PWM-SCM, however, was significantly higher than that generated by Epo. These results suggest that in comparison to the factor(s) in PWM-SCM, Epo stimulates the growth of more mature progenitors.
重组促红细胞生成素（Epo）在含血清和无血清培养中均能刺激小鼠巨核细胞生成，不过需要相对大量的Epo才能为集落生长提供足够的刺激。使用非黏附、非吞噬且去除T细胞的骨髓细胞进行的实验进一步证实了这一观察结果，在这些实验中，Epo刺激了单个巨核细胞以及细胞簇或集落的生长。全平板分析显示，Epo产生的单个巨核细胞和双细胞聚集体数量是商陆丝裂原刺激的脾细胞条件培养基（PWM-SCM）产生数量的两倍。然而，PWM-SCM形成的含四个或更多细胞的集落数量显著高于Epo产生的数量。这些结果表明，与PWM-SCM中的因子相比，Epo刺激更成熟祖细胞的生长。
